您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Early immune response in large yellow croaker (Larimichthys crocea) after immunization with oral vaccine

文献类型： 外文期刊

作者： Zhang, Weibin 1 ; Zhu, Chunhua 1 ; Chi, Hongshu 4 ; Liu, Xiaodong 4 ; Gong, Hui 4 ; Xie, Anhua 4 ; Zheng, Weiqiang 6 ; Ch 1 ;

作者机构： 1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China

2.Fujian Normal Univ, Prov Univ Key Lab Cellular Stress Response & Meta, Coll Life Sci, Fuzhou 350117, Peoples R China

3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Peoples R China

4.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Peoples R China

5.Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China

6.Ningde Fufa Fisheries Co Ltd, State Key Lab Large Yellow Croaker Breeding, Ningde 352103, Fujian, Peoples R China

关键词： Oral vaccine; Mesoporous silica nanoparticles; Immune response; Cytokines

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：1.951； 五年影响因子：1.924 ）

ISSN： 0890-8508

年卷期： 2021 年 56 卷

页码：

收录情况： SCI

摘要： Mesoporous silica nanoparticles (MSNs) have been used in the field of biomedicine as antigen carriers and adjuvants for protective antigens. In the present study, an oral nanovaccine against Vibrio alginolyticus was prepared employing MSNs as carriers. The uptake of the dihydrolipoamide dehydrogenase (DLDH) antigens in the intestine of large yellow croaker was evaluated using an immunohistochemistry assay. Additionally, the effects of the nanovaccine on the early immune response in large yellow croaker were investigated via oral vaccination. The presence of the antigens was detected in the mucosa and lamina propria of the foregut, midgut, and hindgut of large yellow croaker at 3 h following oral immunization. The expression levels of cytokines (i.e., lysozyme, IFN-?, IFITM, TNF-?, IL-1?, IL-2, IL-4, IL-10, and IL-13) in the intestine, spleen, and head kidney tissues of large yellow croaker before and after the immune challenge were determined via RT-qPCR assay. The obtained results revealed that the expression levels of lysozyme, IFN-?, IFITM, TNF-?, IL-1?, IL-2, IL-4, IL-10, and IL-13 in the intestine and head kidney of the vaccinated large yellow croaker, as well as the expression of lysozyme, IL-1?, and IL-10 in the spleen, exhibited time-dependent oscillation regulation patterns. Notably, the nanovaccine immunization could induce early (6 h) and high expression of IFN-? in the spleen and kidney tissues after the bacterial infection. The current study supplements the available data on the early immune response to fish nanovaccines. It also provides a valuable theoretical basis for the future development of large yellow croaker oral vaccines.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台