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A predominant isoform of fructokinase, HbFRK2, is involved in Hevea brasiliensis (para rubber tree) latex yield and regeneration

文献类型： 外文期刊

作者： Fang, Pingchang 1 ; Long, Xiangyu 2 ; Fang, Yongjun 2 ; Chen, Hua 1 ; Yu, Ma 1 ;

作者机构： 1.Southwest Univ Sci & Technol, Sch Life Sci & Engn, Mianyang 621010, Sichuan, Peoples R China

2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou 571101, Hainan, Peoples R China

关键词： Latex regeneration; Fructose phosphorylation; Fructokinase; Expression profiles; Enzymatic properties

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：3.72； 五年影响因子：3.966 ）

ISSN： 0981-9428

年卷期： 2021 年 162 卷

页码：

收录情况： SCI

摘要： Fructokinase (FRK) mediates fructose phosphorylation to regulate the carbon flow and its assignment to sink tissues. Out of five HbFRKs in the genome of the rubber tree, three (HbFRK1-3) that were highly expressed in latex (cytoplasm of laticifers) were isolated and examined. According to phylogenetic analysis and intracellular location experiment, both HbFRK2 and HbFRK3 were highly possible to be expressed in cytosol, while HbFRK1 was in plastid. As the predominant isoform in laticifers, HbFRK2 had the highest transcripts, followed by HbFRK3 and HbFRK1. In enzymatic function, HbFRK2 also showed the highest affinity for fructose. To examine the roles of FRKs in latex yield and regeneration, changes in HbFRKs were examined when latex outflow from the trees were increased through two experimental interventions. In the first approach, tapping was initiated on previously untapped trees, resulting in latex yield increasing with consecutive tapping at the initial stage before it stabilized. In the second approach, latex yield from trees that were already in regular tapping was stimulated by treatment with the ethylene-based yield stimulant, ethephon. Using either method to induce an increase in latex yield, the abundance of HbFRK2 and HbFRK3 in transcripts, was increased. This development, which was especially marked in HbFRK2, may reflect a strengthening of glycolysis to meet the carbon flux and energy demands for increased rubber biosynthesis to replace rubber lost in the increased latex yield. Our results, therefore, suggest that HbFRK2 plays a critical role in fructose catabolism to facilitate rubber regeneration in the commercially exploited rubber tree.

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