文献类型： 外文期刊

作者： Xia Wei ¹ ; Wei Qiaoli ¹ ; Liu Mingzhi ¹ ; Liu Chuang ¹ ; Zhao Qianqian ¹ ; Chen Xiaoyu ³ ; Zhu Zhiwei ³ ; Fang Xiaohuan; ¹ ;

作者机构： 1.Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China

2.Southwest Minzu Univ, Coll Life Sci & Technol, Chengdu, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou, Peoples R China

4.China Agr Univ, Coll Anim Sci & Technol, Beijing, Peoples R China

5.Res Ctr Cattle & Sheep Embryo Engn Tech Hebei Pro, Baoding, Peoples R China

关键词： Fixed-time artificial insemination; pregnancy; transcriptome; corpus luteum

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：1.487； 五年影响因子：1.204 ）

ISSN： 1049-5398

年卷期：

页码：

收录情况： SCI

摘要： Utilization of female livestock can be optimized by application of Fixed-Time Artificial Insemination (FTAI), which plays an important role in large-scale livestock production. However, molecular mechanism of FTAI affecting reproductive performance remains unclear. To investigate the effects of FTAI on corpus luteum in 16 and 25 days of pregnancy gilts, 12 pregnancy gilts were selected from Altrenogest + PMSG + GnRH (APG) group and control group. The number of left and right CL in APG group were significantly higher than control (p < 0.05). Furthermore, result showed the number of differentially expressed genes between 16APG group and 16 C group was obviously larger than that between 25APG and 25 C group (2394 vs. 1476). Up regulated genes in APG were mainly associated with cytokine production and secretion, cell communication and transport (day 16) and angiogenesis, cell cycle and cell-cell signaling (day 25). The expression of differentially expressed genes (RPL10, CYP17A1, DCN, IL18, LDLR and PRLR) in luteal tissue were verified by real-time PCR. In summary, APG program significantly improve reproductive efficiency of gilts through up regulation of cytokine production/secretion, cell communication and transport in Day 16 pregnancy and angiogenesis, cell cycle and cell-cell signaling at Day 25 pregnancy in porcine.