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Transcriptomic analysis to elucidate the effects of high stocking density on grass carp (Ctenopharyngodon idella)

文献类型： 外文期刊

作者： He, Yan 1 ; Yu, Hongyan 1 ; Zhao, Honggang 4 ; Zhu, Hua 5 ; Zhang, Qingjing 5 ; Wang, Anqi 1 ; Shen, Yubang 2 ; Xu, Xiaoy 1 ;

作者机构： 1.Shanghai Ocean Univ, Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Genet Resources, Shanghai, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China

3.Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai, Peoples R China

4.Cornell Univ, Dept Nat Resources, Fernow Hall, Ithaca, NY 14853 USA

5.Beijing Fisheries Res Inst, Beijing Key Lab Fishery Biotechnol, Beijing 100068, Peoples R China

关键词： Ctenopharyngodon idella; High stocking density; Transcriptome; Immune function; Metabolism

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background: Grass carp (Ctenopharyngodon idella) is one of the most widely cultivated fishes in China. High stocking density can reportedly affect fish growth and immunity. Herein we performed PacBio long-read single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing to evaluate the effects of high stocking density on grass carp transcriptome. Results: SMRT sequencing led to the identification of 33,773 genes (14,946 known and 18,827 new genes). From the structure analysis, 8,009 genes were detected with alternative splicing events, 10,219 genes showed alternative polyadenylation sites and 15,521 long noncoding RNAs. Further, 1,235, 962, and 213 differentially expressed genes (DEGs) were identified in the intestine, muscle, and brain tissues, respectively. We performed functional enrichment analyses of DEGs, and they were identified to be significantly enriched in nutrient metabolism and immune function. The expression levels of several genes encoding apolipoproteins and activities of enzymes involved in carbohydrate enzymolysis were found to be upregulated in the high stocking density group, indicating that lipid metabolism and carbohydrate decomposition were accelerated. Besides, four isoforms of grass carp major histocompatibility complex class II antigen alpha and beta chains in the aforementioned three tissue was showed at least a 4-fold decrease. Conclusions: The results suggesting that fish farmed at high stocking densities face issues associated with the metabolism and immune system. To conclude, our results emphasize the importance of maintaining reasonable density in grass carp aquaculture.

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[1]Transcriptomic analysis to elucidate the effects of high stocking density on grass carp (Ctenopharyngodon idella). He, Yan,Yu, Hongyan,Wang, Anqi,Xu, Xiaoyan,Li, Jiale,He, Yan,Yu, Hongyan,Shen, Yubang,Xu, Xiaoyan,Li, Jiale,He, Yan,Xu, Xiaoyan,Li, Jiale,Zhao, Honggang,Zhu, Hua,Zhang, Qingjing. 2021

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