文献类型: 外文期刊
作者: Gou, Jinlin 1 ; Sang, Xuelian 1 ; Liu, Liqin 3 ; Cao, Jiasui 1 ; Liu, Yao 1 ; Ren, Ci 1 ; Zhang, Zhixin 1 ; Jue, Dengwei 1 ; Shi, Shengyou 2 ;
作者机构: 1.Chongqing Univ Arts & Sci, Inst Special Plants, Coll Smart Agr, Chongqing Key Lab Germplasm Innovat Special Aromat, Yongchuan 402160, Peoples R China
2.Sanya Inst Breeding & Multiplicat, Coll Breeding & Multiplicat, Natl Key Lab Trop Crop Breeding, Sanya 572025, Peoples R China
3.Chinese Acad Trop Agr Sci, Key Lab Trop Fruit Biol, Minist Agr, South Subtrop Crops Res Inst, Zhanjiang 524091, Peoples R China
关键词: Longan; CONSTANS family; Flower induction; Functional analysis
期刊名称:BMC PLANT BIOLOGY ( 影响因子:4.8; 五年影响因子:5.4 )
ISSN: 1471-2229
年卷期: 2025 年 25 卷 1 期
页码:
收录情况: SCI
摘要: Longans are among the most economically important subtropical fruits. Its flowering is sensitive to the photoperiod, and flowering time has a significant influence on yield and quality. CONSTANS-like (COL) gene plays a key role in regulating induced flowering in longans. However, the specific role of the COL gene family in the regulation of flowering remains unknown. In this study, 10 DlCOL genes were identified in longans using comprehensive bioinformatics analysis and named based on their physical chromosomal locations. Phylogenetic tree analysis showed that DlCOL genes were divided into three subfamilies, each with a conserved domain. When combined with collinearity analysis, we found DlCOL genes were more closely related to COL genes of dicotyledons. DlCOL family genes are differentially expressed in various longan organs, with DlCOL1, DlCOL3, and DlCOL9 expressed in all organs, with the highest expression levels in floral buds. In the differential expression at different flowering induction stages of 'Sijimi' ('SJ') or 'Shixia' longan ('SX'), DlCOL4 expression was upregulated by 3-fold at the "T1-T2" flowering induction stage in 'SJ', but there was no expression during the three flowering induction stages in 'SX'. Subcellular localization analysis indicated that DlCOL4 is localized in the nucleus. Heterologous transformation of Arabidopsis indicated that DlCOL4 can negatively regulate flowering in transgenic plants. The qRT-PCR (Quantitative real-time PCR) results related to flowering genes indicated that DICOL4 may inhibit flowering by interacting with AtTFL and AtCOL. This study demonstrates the potential functional role of the DlCOL gene and the key role of DlCOL4 in regulating longan flowering.
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