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Insights into the correlations between the size of starch at nano- to microscale and its functional properties based on asymmetrical flow field-flow fractionation

文献类型： 外文期刊

作者： Song, Tiange 1 ; Zhang, Wenhui 1 ; Chen, Xue 3 ; Zhang, Aixia 4 ; Guo, Suna 1 ; Shen, Shigang 1 ; Li, Huili 3 ; Dou, Haiyang 1 ;

作者机构： 1.Hebei Univ, Coll Chem & Environm Sci, Key Lab Analyt Sci & Technol Hebei Prov, Baoding 071002, Peoples R China

2.Hebei Univ, Affiliated Hosp, Baoding 071000, Peoples R China

3.Hebei Univ, Sch Basic Med Sci, Key Lab Pathogenesis Mech & Control Inflammatory, Baoding 071000, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Shijiazhuang 050035, Hebei, Peoples R China

5.Hebei Univ, Coll Qual & Tech Supervis, Natl & Local Joint Engn Res Ctr Metrol Instrument, Baoding 071000, Peoples R China

关键词： Starch; Functional property; Asymmetrical flow field-flow fractionation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2021 年 193 卷

页码：

收录情况： SCI

摘要： In this study, the starches were isolated from three botanical sources (i.e., rice, sweet potato, and lotus seed). The size distributions of starch granules and molecules were determined by asymmetrical flow field-flow fractionation (AF4), and compared with those measured from optical microscopy (OM) and dynamic light scattering (DLS). Furthermore, the starches were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). AF4 coupled online with UV-visible, multiangle light scattering (MALS), and differential refractive index (dRI) detectors (AF4-UV-MALS-dRI) was employed for the investigation of the digestion and retrogradation properties of starches. Meanwhile, the relationships between the size of starch at nano- to microscale and its functional properties (i.e., digestibility, retrogradation, and thermal properties) were studied by Pearson correlation analysis. AF4-UV-MALS-dRI was proved to be a rapid and gentle method for the separation and size characterization of starches at both micro- and nano-molecule levels. Moreover, it was demonstrated that AF4-UVMALS-dRI is a useful tool for the monitoring of the digestion and retrogradation properties of starches. The results suggested that the sizes of starch granules and molecules were to some extent correlated with their thermal properties and digestibility, but not with retrogradation property.

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