您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genetic diversity of rice sheath blight in different geographical regions of China

文献类型： 外文期刊

作者： Tian, Li 1 ; Wang, Chun 2 ; Li, Hailiang 2 ; Sun, Haitian 2 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Informat & Elect Engn, Daqing 163319, Peoples R China

2.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Zhanjiang 524000, Peoples R China

关键词： Rice Sheath Blight; RAPD Marker; Rhizoctonia solani AG1-IA; China

期刊名称：CELLULAR AND MOLECULAR BIOLOGY （ 影响因子：1.6； 五年影响因子：1.6 ）

ISSN： 0145-5680

年卷期： 2022 年 68 卷 10 期

页码：

收录情况： SCI

摘要： Rhizoctonia solani AG1-IA (Thandfephorus cucumeris teleomorph) is the causative agent of rice sheath blight in Chinese paddy fields. Due to the importance of the disease and the lack of comprehensive information on the genetic structure of the fungus populations, 25 isolates collected from Hubei, Sichuan, Anhui, and Jiangsu provinces, and southern China's Yangtze River basin were examined for morphological characteristics, growth rate, and genetic diversity of this pathogen. The anastomosis group determination test results showed that all isolates belong to the AG1-IA anastomosis group. To quickly diagnose and confirm the anastomosis group of isolates, ten isolates along with AG1-IA and AGA standard isolates were examined by specific primers AG1-IA. A 256bp band was amplified in all of them. The results of the growth velocity study divided the isolates into two groups' fast growth (68% of isolates) and slow growth (32% of isolates). The genetic diversity of 25 isolates was assessed using the RAPD marker. Among the 20 primers, bands from seven primers ranging from 250 to 5,000bp were performed using the Jaccard similarity coefficient and UPGMA method by data cluster analysis of NTSYS-pc software. The cluster analysis results divided the isolates into two groups with a similarity level of 36%, which corresponded to the grouping of isolates into two groups of fast growth and slow growth. At the level of 80% similarity, the isolates were divided into 23 groups, which indicates the high genetic diversity of these isolates. The results of the molecular analysis showed that isolates belonging to a geographical area do not necessarily have a genetic affinity. This study performed rapid detection of R. solani AG1-IA using specific primers AG1-IA, and evaluation of genetic diversity of rice sheath blight isolates was performed by RAPD marker in this study.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台