In vitro asymbiotic germination of two endemic Chinese terrestrial Orchis seeds and the physiology of their germination response
文献类型： 外文期刊
作者： Wang, R. X. 2 ; Li, Y. J. 2 ; He, M. G. 3 ; Song, S. Q. 1 ;
作者机构： 1.Chinese Acad Sci, Inst Bot, Grp Seed Physiol & Biotechnol, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Grp Seed Biol, Mengla 666303, Yunnan, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Hainan, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
期刊名称：SEED SCIENCE AND TECHNOLOGY （ 影响因子：0.905； 五年影响因子：0.996 ）
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收录情况： SCI
摘要： Orchis diantha and O. chusua are endangered orchid species, and their seed germination is sparse in nature. To increase seed germination and protect these species, we successfully established the in vitro asymbiotic germination system of mature seeds.The results showed that 1/2 MS (Murashige and Skoog, 1962) medium supplemented with 6-benzyladenine or activated charcoal increased germination of O. diantha seeds, but had no promoting role for O. chusua seeds. In contrary, supplement of coconut milk to 1/2 MS medium increased the germination of O. chusua seeds, and had no role for O. diantha seeds. After-ripening marginally increased germination of O. diantha seeds, and significantly increased germination of O. chusua seeds. Light decreased germination of O. diantha and O. chusua seeds. There is clearly a difference in germination requirements between O. diantha and O. chusua seeds.
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