The Plasma Membrane H+ ATPase CsPMA2 Regulates Lipid Droplet Formation, Appressorial Development and Virulence in Colletotrichum siamense
文献类型： 外文期刊
作者： Liu, Yu 1 ; Xi, Yitao 2 ; Lv, Yanyu 1 ; Yan, Jingting 1 ; Song, Miao 1 ; Yang, Hong 1 ; Zhang, Yu 1 ; Miao, Weiguo 1 ; Lin, Chunhua 1 ;
作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Sanya Inst Breeding & Multiplicat, Haikou 570228, Peoples R China
2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou 571101, Peoples R China
关键词： Colletotrichum siamense; plasma membrane ATPases; CsPMA2; lipid droplets; appressorium development; pathogenicity
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 24 期
页码：
收录情况： SCI
摘要： Plasma membrane H+-ATPases (PMAs) play an important role in the pathogenicity of pathogenic fungi. Lipid droplets are important storage sites for neutral lipids in fungal conidia and hyphae and can be used by plant pathogenic fungi for infection. However, the relationship between plasma membrane H+-ATPase, lipid droplets and virulence remains unclear. Here, we characterized a plasma membrane H+-ATPase, CsPMA2, that plays a key role in lipid droplet formation, appresorial development and virulence in C. siamense. Deletion of CsPMA2 impaired C. siamense conidial size, conidial germination, appressorial development and virulence but did not affect hyphal growth. Delta CsPMA2 increased the sensitivity of C. siamense to phytic acid and oxalic acid. CsPMA2 was localized to lipids on the plasma membrane and intracellular membrane. Deletion of CsPMA2 significantly inhibited the accumulation of lipid droplets and significantly affected the contents of some species of lipids, including 12 species with decreased lipid contents and 3 species with increased lipid contents. Furthermore, low pH can inhibit CsPMA2 expression and lipid droplet accumulation. Overall, our data revealed that the plasma membrane H+-ATPase CsPMA2 is involved in the regulation of lipid droplet formation and affects appressorial development and virulence in C. siamense.
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