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Effects of the skp1 gene of the SCF complex on lipid metabolism and response to abiotic stress in Chlamydomonas reinhardtii

文献类型： 外文期刊

作者： Deng, Xiao Dong 1 ; Wang, Meng 2 ; Liu, Si Hang 2 ; Xu, Dian Long 2 ; Fei, Xiao Wen 1 ;

作者机构： 1.Hainan Med Univ, Sch Basic Med & Life Sci, Key Lab Trop Transnatl Med, Minist Educ, Haikou, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Peoples R China

3.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan P, Haikou, Peoples R China

4.Hainan Prov Key Lab Funct Components Res & Utiliza, Haikou, Peoples R China

5.CATAS, Zhanjiang Expt Stn, Zhanjiang, Peoples R China

关键词： SCF(SKP1-Cullin1-F-box) complex; Skp1; E3 ubiquitin ligase; Chlamydomonas reinhardtii; lipid metabolism; abiotic stress

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： SKP1 (S-phase kinase-associated protein 1) is a key member of the SCF (SKP1-Cullin1-F-box) E3 ligase complex. The SCF complex is involved in regulating various levels of plant physiology, including regulation of cellular signaling and response to abiotic stresses. While the function of SKP1 in plants is well known, its function in algae remains poorly understood. In this study, we investigated the role of the Chlamydomonas reinhardtii skp1 gene using RNAi interference and overexpression approaches. Subcellular localization of SKP1 was performed by transient expression in onion epidermal cells. For abiotic stress assays, the growth of skp1 RNAi and overexpression recombinant strains was examined under conditions of high osmolality (sorbitol), high salinity (NaCl) and high temperature (37 degrees C). Our results showed that skp1 silencing significantly reduced oil accumulation by 38%, whereas skp1 overexpressing led to a 37% increase in oil content, suggesting that skp1 plays a crucial role in regulating oil synthesis and may influence lipid accumulation by regulating photosynthetic carbon flux partitioning. Subcellular localization analysis revealed that skp1 was predominantly localized within the nucleus. Furthermore, our results showed that SKP1 responds to abiotic stresses. Under sorbol and NaCl stress conditions, RNAi interference strains exhibited better growth than controls; however, their growth was comparatively impaired under 37 degrees C stress compared to controls. On the other hand, overexpression strains showed weaker growth under sorbol and NaCl stress but were more tolerant to 37 degrees C heat stress. These results illustrate the functional diversity of SKP1 in Chlamydomonas. This study provides an important complement for lipid metabolism and abiotic stress regulation in microalgae.

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