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One-step creation of CMS lines using a BoCENH3-based haploid induction system in Brassica crop

文献类型： 外文期刊

作者： Han, Fengqing 1 ; Zhang, Xiaoli 2 ; Liu, Yuxiang 1 ; Liu, Yumei 1 ; Zhao, Hong 4 ; Li, Zhansheng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing, Peoples R China

2.Tianjin Acad Agr Sci, State Key Lab Vegetable Biobreeding, Tianjin, Peoples R China

3.Hunan Agr Univ, Engn Res Ctr Hort Crop Germplasm Creat & New Varie, Key Lab Vegetable Biol Hunan Prov, Minist Educ, Changsha, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr BVRC, Natl Engn Res Ctr Vegetables, Beijing, Peoples R China

期刊名称：NATURE PLANTS （ 影响因子：18.0； 五年影响因子：18.6 ）

ISSN： 2055-026X

年卷期： 2024 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Heterosis utilization in a large proportion of crops depends on the use of cytoplasmic male sterility (CMS) tools, requiring the development of homozygous fertile lines and CMS lines1. Although doubled haploid (DH) technology has been developed for several crops to rapidly generate fertile lines2,3, CMS lines are generally created by multiple rounds of backcrossing, which is time consuming and expensive4. Here we describe a method for generating both homozygous fertile and CMS lines through in vivo paternal haploid induction (HI). We generated in-frame deletion and restored frameshift mutants of BoCENH3 in Brassica oleracea using the CRISPR/Cas9 system. The mutants induced paternal haploids by outcrossing. We subsequently generated HI lines with CMS cytoplasm, which enabled the generation of homozygous CMS lines in one step. The BoCENH3-based HI system provides a new DH technology to accelerate breeding in Brassica and other crops.

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