Characterization of Two Novel cry8 Genes from Bacillus thuringiensis Strain BT185
文献类型： 外文期刊
作者： Shu, Changlong 1 ; Yu, Hong 1 ; Wang, Rongyan 2 ; Fen, Shuliang 2 ; Su, Xudong 1 ; Huang, Dafang 3 ; Zhang, Jie 1 ; Song, 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Beijing, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Beijing, Peoples R China
3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China
关键词： conserved block
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
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年卷期：
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收录情况： SCI
摘要： Two novel cry8-type genes, cry8Ea1 and cry8Fa1, obtained from a Holotrichia parallela-specific Bacillus thuringiensis strain, BT185, were characterized. Findings showed that cry8Ea1 and cry8Fa1 encoded polypeptides of 1164 and 1174 amino acid residues, respectively. The deduced amino acid sequences of both Cry8Ea1 and Cry8Fa1 polypeptides are the most similar to that of Cry8Ba1. Eight conserved blocks (blocks 1-8) exist in Cry8Ea1 and Cry8Fa1 polypeptides compared with known Cry proteins. Cry8Ea1 and the Cry8Fa1 toxins could form spheric crystals when they were expressed in the acrystalliferous mutant strain HD73(-). The spores and crystals from the recombinant strain containing cry8Ea1 were toxic to Holotrichia parallela, with an LC50 of 0.0875 x 10(8) colony-forming units (CFU)/g. However, Cry8Fa1 expressed in the recombinant strain was not toxic to H. parallela, Anomala corpulenta, or H. oblita.
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