您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Proteomic analysis of Zhangzagu3 (Setaria italica) and its parents based on iTRAQ technique

文献类型： 外文期刊

作者： Song, Xiao-Qing 1 ; Zhao, Yan 2 ; Weng, Qiao-yun 2 ; Yuan, Jin-Cheng 2 ; Dong, Zhi-Ping 3 ; Zhao, Zhi-Hai 4 ; Liu, Yin 1 ;

作者机构： 1.Hebei North Univ, Basic Med Coll, Dept Biol, Zhangjiakou, Peoples R China

2.Hebei North Univ, Coll Agr & Forestry, Dept Plant Protect, Zhangjiakou, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Dept Millet Res Ctr, Shijiazhuang, Hebei, Peoples R China

4.Zhangjiakou Acad Agr Sci, Dept Millet Res Ctr, Zhangjiakou, Peoples R China

5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China

关键词： proteomic analysis; iTRAQ; heterosis; Zhangzagu3; hybrid millet

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2018 年 32 卷 6 期

页码：

收录情况： SCI

摘要： Zhangzagu3, derived from Zhangzagu3fu and A2 (photoperiod- and thermo-sensitive genic male-sterile line) is well-known for its strong adaptability, good stability, disease, and lodging resistance. To evaluate the parental contributions to its elite traits, isobaric tags for relative and absolute quantitation (iTRAQ) and mass spectrometry (MS) were used to analyze the protein expression profile of these three lines. Enrichment analysis of differentially expressed proteins (DEPs) was performed through GO term and KEGG pathway. A total of 4015 proteins were detected, among which 504/390 DEPs between Zhangzagu3 and the female/male parent, respectively. The cluster analysis using Cluster 3.0 showed that the DEP expression profiles of Zhangzagu3 were closer to those of the male parent. GO functional classification divided the DEPs into three functional categories (cellular component, biological process and molecular function) and 71 subfamilies, among which three subfamilies had 100% difference between Zhangzagu3 and its parents. KEGG metabolic pathway exhibited significant enrichment of differentially expressed genes (DEGs) in Carbon metabolism (mainly Carbon fixation, Glycolysis and Gluconeogenesis), Photosynthesis and Oxidative phosphorylation. qRT-PCR verification confirmed the differential expression data and revealed that the expression level of 17 selected genes (such as putative receptor-like protein kinase, copper chaperone for superoxide dismutase etc.) was higher in Zhangzagu3 than that in its male parent, but significantly lower than that in A2. Thus, A2 can potentially optimize the agricultural qualities of Zhangzagu3. Taken together, we hope that this study provides valuable information for the further utilization and study on the molecular mechanisms of heterosis in hybrid millet.A

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台