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Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

文献类型： 外文期刊

作者： Xu, Huibin 1 ; Jiang, Jiahuang 1 ; Lian, Ling 1 ; Wu, Fangxi 1 ; Zheng, Yanmei 1 ; Zhu, Yongsheng 1 ; Jiang, Minrong 1 ;

作者机构： 1.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350019, Fujian, Peoples R China

2.Minist Agr & Affairs, Key Lab Germplasm Innovat & Mol Breeding Hybrid R, Incubator Natl Key Lab Germplasm Innovat & Mol Br, Fuzhou, Fujian, Peoples R China

3.Minist Sci & Technol, Fuzhou Branch, Natl Rice Improvement Ctr China, Fujian Engn Lab Crop Mol Breeding,Fujian Key Lab, Fuzhou, Fujian, Peoples R China

4.State Key Lab Hybrid Rice, Fuzhou, Fujian, Peoples R China

5.Natl Rice Engn Lab, Fuzhou, Fujian, Peoples R China

6.Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou, Fujian, Peoples R China

关键词： 2D-PAGE; artificial ageing; iTRAQ; longevity; rice

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期：

页码：

收录情况： SCI

摘要： Seed longevity is a very important characteristic controlling seed quality. However, the mechanism underlying this characteristic is poorly understood. In this study, 'FH7185', a storable rice variety, the germination rate of which was 66.63%, much higher than the control after artificial ageing under relative humidity 88% and 42 degrees C for 21 days. Different methods were applied to reveal the involvement of proteins during ageing of rice seeds. In total, 35 differential proteins were identified from 2D-PAGE, and 3,719 proteins from iTRAQ analysis. A comparison of these two methods showed that not all protein types could be detected on the 2D-PAGE gels, and the dynamic range was somewhat limited. So the comprehensive proteome map from the iTRAQ analysis was used to identify the quantitatively regulated proteins that played roles in seed longevity, and found that the most marked change was the increased abundance of many metabolic enzymes, especially the ones involved in alpha-linolenic acid metabolism in the embryos during ageing. OsLOX2 and OsLOX3 had a negative effect on seed longevity, which were lower in FH7185 than the control. The dehydrogenase (LOC_Os07g44430) which played a major catalytic role in lipid peroxidation, also changed significantly during ageing. Therefore, OsLOX2, OsLOX3 and Loc_OS 07G44430 may be involved in the regulation of seed longevity with a synergistic effect.

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