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Exploration of the temporal-spatial expression pattern and DNA methylation-related regulation of the duck telomerase reverse transcriptase gene

文献类型： 外文期刊

作者： Yuan, Taoyan 1 ; Zhao, Wanqiu 1 ; Niu, Yifan 4 ; Fu, Yan 1 ; Lu, Lizhi 3 ; Niu, Dong 2 ;

作者机构： 1.Zhejiang Univ, Coll Anim Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang A&F Univ, Coll Anim Sci & Technol, Hangzhou, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, 145 Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China

5.Minist Agr China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

关键词： duck; TERT; DNA methylation; telomerase activity; telomere length

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

ISSN： 0032-5791

年卷期： 2019 年 98 卷 8 期

页码：

收录情况： SCI

摘要： Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase that adds TTAGGG repeats to the 3'-overhang of telomeres. In the present study, we detected that the duck TERT (dTERT) gene was highly expressed in small intestine and kidney, followed by heart, leg muscle, spleen, pancreas, gonad, and liver at neonatal stage. From embryonic to neonatal stage, the highest dTERT mRNA in liver appeared at stage E19 (19 days at embryonic stage), while for the leg muscle the maximum expression occurred at E26. We also measured the relative telomerase activity (RTA) and relative telomere length (RTL) in the examined tissues and found that the changed tendency of RTA and RTL was not very consistent with that of TERT. In silico analysis revealed that there were three CpG islands (S1, S2, and S3) within the 5' regulatory region of the dTERT gene. Bisulfite sequencing PCR (BSP) assay showed that liver (D7, 7 days after birth) which expressed significantly lower dTERT mRNA had an obviously higher methylation level of S1 compared with small intestine (D7) or liver (E19). Quantitative real-time PCR analysis revealed that the expression of DNA methyltransferase DNMT1 in liver (D7) was significantly higher than that in small intestine (D7) or in liver (E19). In vitro, dTERT expression was upregulated and the methylation status of S1 decreased in both duck embryonic fibroblasts and small intestinal epithelial cells following treatment with the demethylation reagent, 5-aza-2'-deoxycytidine (5-aza-dC), further suggesting that dTERT is epigenetically regulated by DNA methylation. This work lays a solid foundation for further study of TERT function and regulation in avian species.

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