文献类型： 外文期刊

作者： Wang, Rang Jian ¹ ; Gao, Xiang Feng ¹ ; Yang, Jun ¹ ; Kong, Xiang Rui ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Inst Tea, 1 Hutouyang Rd, Fuan 355015, Fujian, Peoples R China

2.Natl Ctr Tea Improvement, Fujian Branch, 1 Hutouyang Rd, Fuan 355015, Fujian, Peoples R China

关键词： tea plant; timing of spring bud flush; quantitative trait loci; genome-wide association study; marker-assisted selection

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 37 期

页码：

收录情况： SCI

摘要： The timing of spring bud flush (TBF) is of economic importance for tea plant (Camellia sinensis) breeding. We employed a genome-wide association study (GWAS) to identify favorable single nucleotide polymorphism (SNP) allelic variations as well as candidate genes that control TBF of C. sinensis using specific-locus-amplified fragment sequencing (SLAF-seq) in a diversity panel comprising 151 tea plant germplasm resources. GWAS analysis revealed 26 SNPs associated with TBF in three years, and we eventually identified a final significant SNP for TBF. To identify candidate genes possibly related to TBF, we screened seven candidate genes within 100 kb regions surrounding the trait-related SNP loci. Furthermore, the favorable allelic variation, the "TT" genotype in the SNP loci, was discovered, and a derived cleaved amplified polymorphism (dCAPS) marker was designed that cosegregated with TBF, which could be used for marker-assisted selection (MAS) breeding in C. sinensis. The results obtained from this study can provide a theoretical and applied basis for the MAS of early breeding in tea plants in the future.