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A laser-induced breakdown spectroscopy-integrated lateral flow strip (LIBS-LFS) sensor for rapid detection of pathogen

文献类型: 外文期刊

作者: Wu, Jing 1 ; Liu, Yong 2 ; Cui, Youwei 1 ; Zhao, Xiaohui 2 ; Dong, Daming 1 ;

作者机构: 1.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Intelligent Equipment Agr, Beijing 100097, Peoples R China

2.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词: Lateral flow strip; Laser-induced breakdown spectroscopy; Pathogen detection; AgxAuy bimetallic nanoparticles; Staphylococcus aureus

期刊名称:BIOSENSORS & BIOELECTRONICS ( 影响因子:10.618; 五年影响因子:9.323 )

ISSN: 0956-5663

年卷期: 2019 年 142 卷

页码:

收录情况: SCI

摘要: Incorporation of new readout methods with established analytical devices allows methodological innovations in analytical sciences. Herein, we present a new sensing platform by combining an ultrasensitive element analyzer, namely the laser-induced breakdown spectroscopy (LIBS) and a lateral flow strip (LFS). AgxAuy bimetallic nanoparticles (Ag(x)Au(y)BNPs) are selected as the labels to deliver the optimal quantitative performance by analyzing the Ag (I) signal from the test (T) line of LFS. For prototypical application in pathogen detection, the LIBS-LFS sensor can achieve a detection limit of 1.6 cfu mL(-1) of Staphylococcus aureus (S. aureus) within 10 min, which is superior to conventional methods. Importantly, the signals of Ag(x)Au(y)BNPs for visual and LIBS analysis are stable and still readable after the detection is finished and the test strip is stored for up to 13 days, suggesting a potential for long-term data preservation. This combination of LIBS with LFS provides a new concept toward integrated nano/analytical devices that can benefit various application scenarios.

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