Two B-box proteins, PpBBX18 and PpBBX21, antagonistically regulate anthocyanin biosynthesis via competitive association with Pyrus pyrifolia ELONGATED HYPOCOTYL 5 in the peel of pear fruit
文献类型： 外文期刊
作者： Bai, Songling 1 ; Tao, Ruiyan 1 ; Yin, Lei 1 ; Ni, Junbei 1 ; Yang, Qinsong 1 ; Yan, Xinhui 1 ; Yang, Feng 1 ; Guo, Xianp 1 ;
作者机构： 1.Zhejiang Univ, Dept Hort, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Prov Key Lab Integrat Biol Hort Plants, Hangzhou 310058, Zhejiang, Peoples R China
3.Minist Agr China, Key Lab Hort Plant Growth Dev & Qual Improvement, Hangzhou 310058, Zhejiang, Peoples R China
4.Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450002, Henan, Peoples R China
5.Gansu Acad Agr Sci, Inst Fruit & Floriculture Res, Lanzhou 730070, Gansu, Peoples R China
关键词： anthocyanin accumulation; BBX proteins; HY5; light signal transduction; MYB10; Pyrus pyrifolia; red pear
期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）
ISSN： 0960-7412
年卷期：
页码：
收录情况： SCI
摘要： Light is indispensable for the accumulation of anthocyanin in the peel of red pear fruit (Pyrus pyrifolia Nakai). ELONGATED HYPOCOTYL 5 (HY5) is considered to be a critical regulator for induction of anthocyanin biosynthesis, but detailed characterization of its regulatory mechanism is needed. In this study, multiple genetic and biochemical approaches were applied to identify the roles of P. pyrifolia HY5 (PpHY5) and two B-box (BBX) proteins, PpBBX18 and PpBBX21, in the transcriptional regulation of PpMYB10. The functions of the two BBX proteins were analyzed in overexpression lines using pear calli-based approaches. On its own PpHY5 was unable to activate downstream genes. The two BBX proteins, PpBBX18 and PpBBX21, physically interacted with PpHY5 and antagonistically regulated anthocyanin biosynthesis in Arabidopsis and pear. PpBBX18 formed a heterodimer with PpHY5 via two B-box domains, in which PpHY5 bound to the G-box motif of PpMYB10 and PpBBX18 provided the trans-acting activity, thus inducing transcription of PpMYB10. PpBBX21 interacted with PpHY5 and PpBBX18 and hampered formation of the PpHY5-PpBBX18 active transcription activator complex, and subsequently repressed anthocyanin biosynthesis. The present results demonstrate the fine-tuned regulation of anthocyanin biosynthesis via transcriptional regulation of PpMYB10 by PpHY5-associated proteins and provide insights into light-induced anthocyanin biosynthesis.
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