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Hybrid de novo genome assembly of Chinese chestnut (Castanea mollissima)

文献类型: 外文期刊

作者: Xing, Yu 1 ; Liu, Yang 2 ; Zhang, Qing 2 ; Nie, Xinghua 2 ; Sun, Yamin 3 ; Zhang, Zhiyong 1 ; Li, Huchen 1 ; Fang, Kefeng 5 ;

作者机构: 1.Beijing Univ Agr, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 7 Beinong Rd, Beijing 102206, Peoples R China

2.Beijing Univ Agr, Coll Plant Sci & Technol, Beijing Key Lab Agr Applicat & New Tech, 7 Beinong Rd, Beijing 102206, Peoples R China

3.Res Ctr Funct Genom & Biochip, 23 Hongda St, Tianjin 300457, Peoples R China

4.Wageningen Univ, Dept Plant Sci, Lab Mol Biol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

5.Beijing Univ Agr, Coll Landscape Architecture, Beijing Collaborat Innovat Ctr Ecoenvironm Improv, 7 Beinong Rd, Beijing 102206, Peoples R China

6.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, 39 E Jieyangdajie, Changli 066600, Peoples R China

7.Chinese Acad Sci, South China Bot Garden, 723 Xingke Rd, Guangzhou 510650, Guangdong, Peoples R China

关键词: Castanea mollissima; genome assembly; annotation; evolution

期刊名称:GIGASCIENCE ( 影响因子:6.524; 五年影响因子:8.702 )

ISSN: 2047-217X

年卷期: 2019 年 8 卷 9 期

页码:

收录情况: SCI

摘要: Background: The Chinese chestnut (Castanea mollissima) is widely cultivated in China for nut production. This plant also plays an important ecological role in afforestation and ecosystem services. To facilitate and expand the use of C. mollissima for breeding and its genetic improvement, we report here the whole-genome sequence of C. mollissima. Findings: We produced a high-quality assembly of the C. mollissima genome using Pacific Biosciences single-molecule sequencing. The final draft genome is similar to 785.53 Mb long, with a contig N50 size of 944 kb, and we further annotated 36,479 protein-coding genes in the genome. Phylogenetic analysis showed that C. mollissima diverged from Quercus robur, a member of the Fagaceae family, similar to 13.62 million years ago. Conclusions: The high-quality whole-genome assembly of C. mollissima will be a valuable resource for further genetic improvement and breeding for disease resistance and nut quality.

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