High Frequency 2n Pollen Formation in Black Poplar (Populus nigra L.) Induced by Colchicine
文献类型： 外文期刊
作者： Xu, Wenting 1 ; Zhang, Yan 1 ; Gu, Liqin 1 ; Zha, Jian 1 ; Wei, Zunzheng 4 ; Zhang, Jinfeng 1 ;
作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol,Minist Educ,Key Lab Fores, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding,Key Lab Genet & Breed, Beijing 100083, Peoples R China
2.Zhejiang A&F Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China
3.Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Key Lab Urban Agr North,Beijing Vegetable Res Ctr, Beijing 100091, Peoples R China
关键词： 2n pollen; colchicine; Populus nigra L.
期刊名称：NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA （ 影响因子：1.444； 五年影响因子：1.363 ）
ISSN： 0255-965X
年卷期： 2019 年 47 卷 3 期
页码：
收录情况： SCI
摘要： Populus nigra L. is one of the most important male genetic donors in populus genetic improvement and tree breeding over the world. Many excellent Populus nigra clones are identified as triploids potentially obtaining by hybridization of 2n pollen and normal oogamete. This study revealed the cytological mechanism of 2n pollen formation in Populus nigra L. for the first time and concluded the best treated combination of colchicine treatment, which obtained 2n pollen with the highest rate reaching at 87.11% (even 100% 2n pollen in some floral buds) which made the polyploid hybridization utilizing 2n pollen in section Aigeiros possible.
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