EFFICIENT, CHEAP AND RELIABLE METHOD FOR THE EXTRACTION OF gDNA FROM THE HARD AND COMPLEX TISSUES OF COCONUT
文献类型： 外文期刊
作者： Iqbal, Amjad 1 ; Yang, Yaodong 1 ; Shah, Farooq 2 ; Shah, Tariq 3 ; Hamayun, Muhammad 4 ; Hussain, Anwar 4 ; Khan, Zaf 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Key Lab Trop Oil Crops Biol, Coconut Res Inst, Wenchang 571339, Hainan, Peoples R China
2.Abdul Wali Khan Univ Mardan, Dept Agr, Mardan, Pakistan
3.Huazhong Agr Univ Wuhan, Dept Econ & Management, Wuhan, Hubei, Peoples R China
4.Abdul Wali Khan Univ Mardan, Dept Bot, Mardan, Pakistan
关键词： gDNA; nucleic acid extraction; coconut; palmacea; hard tissues
期刊名称：FRESENIUS ENVIRONMENTAL BULLETIN （ 影响因子：0.489； 五年影响因子：0.479 ）
ISSN： 1018-4619
年卷期： 2019 年 28 卷 11 期
页码：
收录情况： SCI
摘要： Here we explained a convenient, rapid and cheap method of gDNA extraction from the hard and complex tissues of the coconut. The method has also been verified against betel nut and available vegetables (mint, spring onion, radish, ginger, coriander) at the Coconut Research Institute, Wenchang-China. Extraction of gDNA was carried out through 8 simple steps. The method doesn't require intensive cleaning steps as designated to the commercially available kits. The method efficiently isolated the highly intact gDNA with great purity. This cheap and reliable method has broad future prospects in the field of plant and animal genetics, breeding and pathology.
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