Th1-biased immunoadjuvant effect of the recombinant B subunit of an Escherichia coli heat-labile enterotoxin on an inactivated porcine reproductive and respiratory syndrome virus antigen via intranasal immunization in mice
文献类型： 外文期刊
作者： Su, Fei 1 ; Xu, Lihua 1 ; Xue, Yin 2 ; Li, Junxing 1 ; Fu, Yuan 1 ; Yu, Bin 1 ; Wang, Sai 1 ; Yuan, Xiufang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310002, Zhejiang, Peoples R China
2.Zhejiang Ctr Anim Dis Control, Hangzhou 310020, Zhejiang, Peoples R China
关键词： intranasal adjuvant; porcine reproductive and respiratory syndrome virus antigen; rLTB; Th1
期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）
ISSN： 0916-7250
年卷期： 2019 年 81 卷 10 期
页码：
收录情况： SCI
摘要： Porcine reproductive and respiratory syndrome (PRRS) is one of the major swine diseases responsible for a significant challenge in the global swine industry. The current PRRS inactivated vaccine only confers limited protection against PRRSV. Thus, using an appropriate adjuvant via a suitable administration route may help improve vaccine efficacy. In this study, the recombinant B subunit of the Escherichia coli heat-labile enterotoxin rLTB, was highly expressed in Pichia pastoris, through high-density fermentation. rLTB intranasal adjuvant properties were evaluated on an inactivated PRRS antigen in mice. Compared to the group immunized with solely PRRS antigen, a dose of 50 mu g rLTB remarkably raised antigen-specific IgA antibodies at mucosal sites, and increased serum IgG antibodies, preferentially the IgG2a and IgG2b subclasses. Further, rLTB induced increases in Th1- (IFN-gamma and IL-12) and Th17 (IL-6) cytokine profiles, but had little effect on Th2 cytokine profiles (IL-4 and IL-10). Moreover, there were no overt toxicities associated with intranasal rLTB administration. Our data provide evidence that the rLTB produced by P. pastoris fermentation portrays low toxicity, and its intranasal adjuvant effect involves immune system modulation to a Th1 profile.
- 相关文献
作者其他论文 更多>>
-
Genome-based assessment of antimicrobial resistance of Escherichia coli recovered from diseased swine in eastern China for a 12-year period
作者：Li, Junxing;Yuan, Xiufang;Xu, Lihua;Yu, Bin;Su, Fei;Ye, Shiyi;Chen, Yijie;Chang, Jiang;Wu, Jing;Guan, Chunjiu;Tang, Biao;Chang, Jiang;Yang, Yue;Zhao, Guoping;Tang, Biao;Ma, Jiangang;Zhou, Wei;Zhao, Guoping;Zhao, Guoping
关键词：
Escherichia coli ; swine; antimicrobial resistance;mcr ;tet (X4) -
Neutralizing monoclonal antibodies as effective therapeutics and prophylactics against lethal H10N7 avian influenza infection in a mouse model
作者：Wang, Ping;Fu, Jiamin;Cheng, Linfang;Yan, Sijing;Wu, Han;Liu, Fumin;Yao, Hangping;Wu, Nanping;Wu, Haibo;Wang, Ping;Fu, Jiamin;Cheng, Linfang;Yan, Sijing;Wu, Han;Liu, Fumin;Yao, Hangping;Wu, Nanping;Wu, Haibo;Xu, Lihua
关键词：Avian influenza virus; H10 subtype; monoclonal antibodies; neutralizing; hemagglutination
-
Development of a graphene oxide multilayer quantum dot-based immunochromatographic strip for the ultrasensitive detection of H7 subtype avian influenza viruses
作者：Fu, Jiamin;Wang, Ping;Wu, Han;Cheng, Linfang;Liu, Fumin;Yao, Hangping;Wu, Nanping;Wu, Haibo;Fu, Jiamin;Wang, Ping;Wu, Han;Cheng, Linfang;Liu, Fumin;Yao, Hangping;Wu, Nanping;Wu, Haibo;Yang, Fan;Xu, Lihua
关键词：H7 subtype influenza virus; Immunochromatographic strip; Graphene oxide; Quantum dot; Ultrasensitive detection
-
Genetic and molecular characterization of a novel reassortant H3N2 influenza virus from a sick pig in Eastern China in 2019
作者：Yang, Fan;Cheng, Linfang;Liu, Fumin;Yao, Hangping;Wu, Nanping;Wu, Haibo;Yang, Fan;Cheng, Linfang;Liu, Fumin;Yao, Hangping;Wu, Nanping;Wu, Haibo;Xu, Lihua
关键词：Swine influenza virus; reassortant; Eastern China
-
Rapid detection of avipoxvirus using a fluorescent probe-based multienzyme isothermal amplification assay
作者：Zhu, Yinchu;Huo, Suxin;Chen, Liu;Fu, Yuan;Hua, Jionggang;Yun, Tao;Zhang, Cun;Ni, Zheng;Ye, Weicheng
关键词：avipoxvirus; multienzyme isothermal amplification; nucleic acid detection; rapid assay; clinic diagnostic
-
In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector
作者：Chen, Liu;Zhu, Yinchu;Yun, Tao;Ye, Weicheng;Ni, Zheng;Hua, Jionggang;Fu, Yuan;Zhang, Cun
关键词：Duck enteritis virus; Goose astrovirus; Viral vector; Virus-like particles
-
Portable lab-on-a-chip platform enabling multiplex recombinant enzyme polymerase amplification detection of H5/H7/H10 avian influenza virus subtypes
作者：Liang, Li-guo;Wang, Ping;Fu, Jiamin;Zhu, Linwei;Cheng, Linfang;Liu, Fumin;Wu, Nanping;Yao, Hangping;Wu, Haibo;Liang, Li-guo;Wang, Ping;Fu, Jiamin;Zhu, Linwei;Cheng, Linfang;Liu, Fumin;Wu, Nanping;Yao, Hangping;Wu, Haibo;Liang, Li-guo;Xu, Lihua
关键词：Avian influenza virus; Subtype; Detection; Microfluidic microarray; Recombinant enzyme polymerase amplification
