文献类型： 外文期刊

作者： Wu, Shan ¹ ; Wang, Xin ¹ ; Reddy, Umesh ² ; Sun, Honghe ¹ ; Bao, Kan ¹ ; Gao, Lei ¹ ; Mao, Linyong ¹ ; Patel, Takshay ⁵ ; Or ¹ ;

作者机构： 1.Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14850 USA

2.West Virginia State Univ, Dept Biol, Institute, WV USA

3.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Key Lab Biol & Genet Improvement Hort Crops North, Beijing, Peoples R China

4.Howard Univ, Coll Med, Dept Biochem & Mol Biol, Washington, DC USA

5.North Carolina State Univ, Hort Sci Dept, Raleigh, NC USA

6.USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA

7.Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA

8.Agr Res Org, Dept Vegetable Res, Newe Yaar Res Ctr, Ramat Yishay, Israel

9.Sakata Seed Amer, Ft Myers, FL USA

10.Woodland Res Stn, Sakata Seed Amer, Woodland, CA USA

11.Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL USA

12.Univ Georgia, Dept Hort, Athens, GA 30602 USA

13.USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA USA

14.Syngenta Beijing Innovat Ctr, Beijing, Peoples R China

15.USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA

关键词： watermelon; 'Charleston Gray'; genome sequence; genetic diversity; Citrullus germplasm; genotyping-by-sequencing; genome-wide association study; disease resistance

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2019 年 17 卷 12 期

页码：

收录情况： SCI

摘要： Years of selection for desirable fruit quality traits in dessert watermelon (Citrullus lanatus) has resulted in a narrow genetic base in modern cultivars. Development of novel genomic and genetic resources offers great potential to expand genetic diversity and improve important traits in watermelon. Here, we report a high-quality genome sequence of watermelon cultivar 'Charleston Gray', a principal American dessert watermelon, to complement the existing reference genome from '97103', an East Asian cultivar. Comparative analyses between genomes of 'Charleston Gray' and '97103' revealed genomic variants that may underlie phenotypic differences between the two cultivars. We then genotyped 1365 watermelon plant introduction (PI) lines maintained at the U.S. National Plant Germplasm System using genotyping-by-sequencing (GBS). These PI lines were collected throughout the world and belong to three Citrullus species, C. lanatus, C. mucosospermus and C. amarus. Approximately 25 000 high-quality single nucleotide polymorphisms (SNPs) were derived from the GBS data using the 'Charleston Gray' genome as the reference. Population genomic analyses using these SNPs discovered a close relationship between C. lanatus and C. mucosospermus and identified four major groups in these two species correlated to their geographic locations. Citrullus amarus was found to have a distinct genetic makeup compared to C. lanatus and C. mucosospermus. The SNPs also enabled identification of genomic regions associated with important fruit quality and disease resistance traits through genome-wide association studies. The high-quality 'Charleston Gray' genome and the genotyping data of this large collection of watermelon accessions provide valuable resources for facilitating watermelon research, breeding and improvement.