文献类型： 外文期刊

作者： Cheng, Hongtao ¹ ; Jin, Fenwei ² ; Zaman, Qamar U. ¹ ; Ding, Bingli ¹ ; Hao, Mengyu ¹ ; Wang, Yi ² ; Huang, Yi ¹ ; Wells, ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol Sci & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Wuhan 430062, Hubei, Peoples R China

2.Gansu Acad Agr Sci, Crop Res Inst, Lanzhou 730070, Gansu, Peoples R China

3.John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

关键词： Brassica napus; Dwarf; IAA7; MutMap; Auxin; Tissue culture

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2019 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Background Plant height is one of the most important agronomic traits in many crops due to its influence on lodging resistance and yield performance. Although progress has been made in the use of dwarfing genes in crop improvement, identification of new dwarf germplasm is still of significant interest for breeding varieties with increased yield. Results Here we describe a dominant, dwarf mutant G7 of Brassica napus with down-curved leaves derived from tissue culture. To explore the genetic variation responsible for the dwarf phenotype, the mutant was crossed to a conventional line to develop a segregating F-2 population. Bulks were formed from plants with either dwarf or conventional plant height and subjected to high throughput sequencing analysis via mutation mapping (MutMap). The dwarf mutation was mapped to a 0.6 Mb interval of B. napus chromosome C05. Candidate gene analysis revealed that one SNP causing an amino acid change in the domain II of Bna.IAA7.C05 may contribute to the dwarf phenotype. This is consistent with the phenotype of a gain-of-function indole-3-acetic acid (iaa) mutant in Bna.IAA7.C05 reported recently. GO and KEGG analysis of RNA-seq data revealed the down-regulation of auxin related genes, including many other IAA and small up regulated response (SAUR) genes, in the dwarf mutant. Conclusion Our studies characterize a new allele of Bna.IAA7.C05 responsible for the dwarf mutant generated from tissue culture. This may provide a valuable genetic resource for breeding for lodging resistance and compact plant stature in B. napus.