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Genetic mapping reveals a candidate gene for egusi seed in watermelon

文献类型： 外文期刊

作者： Luan, Feishi 1 ; Fan, Chao 1 ; Sun, Lei 1 ; Cui, Haonan 1 ; Amanullah, Sikandar 1 ; Tang, Lili 1 ; Gao, Peng 1 ;

作者机构： 1.Northeast Agr Univ, Coll Hort & Landscape Architecture, 600 Changjiang St, Harbin 150030, Heilongjiang, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Harbin 150030, Heilongjiang, Peoples R China

3.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Tillage, Harbin 150001, Heilongjiang, Peoples R China

关键词： Egusi seed; Watermelon; Candidate gene; Genetic mapping; Expression analysis

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2019 年 215 卷 11 期

页码：

收录情况： SCI

摘要： Citrullus mucosospermus (egusi watermelon) and Citrullus lanatus (dessert watermelon) are closely related subspecies. Variation in their seed coats underlies a key to understand the major differences in seeds between these two subspecies. Although the genetic model is understood, the candidate genes controlling the egusi seed trait in watermelon are unknown. In this study, a locus that controls the egusi seed trait, WEG6.1, which is located on chromosome 6 of the watermelon genome, was identified. A total of 195 polymorphic primers and 325 F-2 plants were used to construct a primary mapping population and linkage maps. A large population of 758 F-2 plants was used for fine mapping of the eg gene. The candidate gene was mapped to a 130.7 kb region on chromosome 6. Eleven genes were present within the corresponding region of the reference genome. All eleven candidate genes were sequenced, revealing that the CDS of the Cla007520 gene had a 3 bp deletion that resulted in the omission of one amino acid (serine) in C. mucosospermus. In addition, expression of the Cla007520 gene was highest at the early stage of seed formation, exhibiting a downward trend thereafter. The predicted protein of the Cla007520 gene belonged to the CPP protein family, and its amino acid constituents were similar to those of AtTSO1, which is associated with promoting integument cell proliferation in ovule development. This study revealed genes that facilitate the formation of egusi seed and might lead to a breakthrough in seed development and cultivated watermelon domestication.

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