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Specific detection of the novel goose astrovirus using a TaqMan real-time RT-PCR technology

文献类型： 外文期刊

作者： Wan, Chunhe 1 ; Chen, Cuiteng 1 ; Cheng, Longfei 1 ; Fu, Guanghua 1 ; Shi, Shaohua 1 ; Liu, Rongchang 1 ; Chen, Hongme 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

2.Longyan Univ, Fujian Prov Key Lab Prevent & Control Anim Infect, Longyan 364012, Peoples R China

3.Fujian Anim Dis Control Technol Dev Ctr, Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China

关键词： Novel goose astrovirus (N-GoAstV); TaqMan; qRT-PCR; Detection; Transmission

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2019 年 137 卷

页码：

收录情况： SCI

摘要： Recently, a novel goose astrovirus (N-GoAstV) was discovered in China, with the transmission route of N-GoAstV unclear. In this study, we developed a TaqMan-based real-time RT-PCR (qRT-PCR) assay for the detection of N-GoAstV infection. After the optimization of the qRT-PCR assay conditions, the results demonstrated that the lower limit of detection for N-GoAstV was 33.4 copies/mu L. No cross-reactivity was observed with other goose-origin viruses. Intra-assay and inter-assay variability were <= 1.36% and 2.34%, respectively. N-GoAstV was detected in both field samples, embryos and newly hatched goslings by qRT-PCR assay, provided the view that N-GoAstV may be both horizontally and vertically transmitted. The established qRT-PCR method showed high specificity, sensitivity, and reproducibility, which can be used in future investigations on the pathogenesis and epidemiology of N-GoAstV.

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