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PINOID is required for lateral organ morphogenesis and ovule development in cucumber

文献类型： 外文期刊

作者： Liu, Xiaofeng 1 ; Hao, Ning 3 ; Li, Huiyuan 3 ; Ge, Danfeng 4 ; Du, Yalin 1 ; Liu, Renyi 5 ; Wen, Changlong 7 ; Li, Yuhon 1 ;

作者机构： 1.Hunan Agr Univ, Coll Hort & Landscape Architecture, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China

2.China Agr Univ, Beijing Key Lab Growth & Dev Regulat Protected Ve, MOE Joint Int Res Lab Crop Mol Breeding, Beijing 100193, Peoples R China

3.Northeast Agr Univ, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Harbin 150030, Heilongjiang, Peoples R China

4.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China

5.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Fujian, Peoples R China

6.Fujian Agr & Forestry Univ, FAFU UCR Joint Ctr Hort Biol & Metabol, Haixia Inst Sci & Technol, Fuzhou 350002, Fujian, Peoples R China

7.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing Key Lab Vegetable Germplasms Improvement, Beijing 100097, Peoples R China

8.Northwest A&F Univ, Coll Hort, Yangling 712100, Shanxi, Peoples R China

关键词： Auxin; Cucumis sativus L; lateral organs; ovule development; PINOID; polar transport

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2019 年 70 卷 20 期

页码：

收录情况： SCI

摘要： Lateral organ development is essential for cucumber production. The protein kinase PINOID (PID) participates in distinct aspects of plant development by mediating polar auxin transport in different species. Here, we obtained a round leaf (rl) mutant that displayed extensive phenotypes including round leaf shape, inhibited tendril outgrowth, abnormal floral organs, and disrupted ovule genesis. MutMap(+) analysis revealed that rl encodes a cucumber ortholog of PID (CsPID). A non-synonymous single nucleotide polymorphism in the second exon of CsPID resulted in an amino acid substitution from arginine to lysine in the rl mutant. Allelic testing using the mutant allele C356 with similar phenotypes verified that CsPID was the causal gene. CsPID was preferentially expressed in young leaf and flower buds and down-regulated in the rl mutant. Subcellular localization showed that the mutant form, Cspid, showed a dotted pattern of localization, in contrast to the continuous pattern of CsPID in the periphery of the cell and nucleus. Complementation analysis in Arabidopsis showed that CsPID, but not Cspid, can partially rescue the pid-14 mutant phenotype. Moreover, indole-3-acetic acid content was greatly reduced in the rl mutant. Transcriptome profiling revealed that transcription factors, ovule morphogenesis, and auxin transport-related genes were significantly down-regulated in the rl mutant. Biochemical analysis showed that CsPID physically interacted with a key polarity protein, CsREV (REVOLUTA). We developed a model in which CsPID regulates lateral organ morphogenesis and ovule development by stimulating genes related to auxin transport and ovule development.

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