Identification of a Major QTL (qRRs-10.1) That Confers Resistance to Ralstonia solanacearum in Pepper (Capsicum annuum) Using SLAF-BSA and QTL Mapping
文献类型： 外文期刊
作者： Du, Heshan 1 ; Wen, Changlong 1 ; Zhang, Xiaofen 1 ; Xu, Xiulan 1 ; Yang, Jingjing 1 ; Chen, Bin 1 ; Geng, Sansheng 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, BVRC, Natl Engn Res Ctr Vegetables, Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China
关键词： Capsicum annuum; Ralstonia solanacearum; SLAF-seq; QTL; linkage mapping; rootstock
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2019 年 20 卷 23 期
页码：
收录情况： SCI
摘要： The soilborne pathogen Ralstonia solanacearum is the causal agent of bacterial wilt (BW), a major disease of pepper (Capsicum annuum). The genetic basis of resistance to this disease in pepper is not well known. This study aimed to identify BW resistance markers in pepper. Analysis of the dynamics of bioluminescent R. solanacearum colonization in reciprocal grafts of a resistant (BVRC 1) line and a susceptible (BVRC 25) line revealed that the resistant rootstock effectively suppressed the spreading of bacteria into the scion. The two clear-cut phenotypic distributions of the disease severity index in 440 F2 plants derived from BVRC 25 x BVRC 1 indicated that a major genetic factor as well as a few minor factors that control BW resistance. By specific-locus amplified fragment sequencing combined with bulked segregant analysis, two adjacent resistance-associated regions on chromosome 10 were identified. Quantitative trait (QTL) mapping revealed that these two regions belong to a single QTL, qRRs-10.1. The marker ID10-194305124, which reached a maximum log-likelihood value at 9.79 and accounted for 19.01% of the phenotypic variation, was located the closest to the QTL peak. A cluster of five predicted R genes and three defense-related genes, which are located in close proximity to the significant markers ID10-194305124 or ID10-196208712, are important candidate genes that may confer BW resistance in pepper.
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