Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants
文献类型： 外文期刊
作者： Wang, Da-Cheng 1 ; Jiang, Chun-Hao 1 ; Zhang, Li-Na 1 ; Chen, Lin 1 ; Zhang, Xiao-Yun 2 ; Guo, Jian-Hua 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect,Dept Plant Pathol, Key Lab Monitoring & Management Crop Dis & Pest I, Engn Ctr Bioresource Pesticide Jiangsu Prov,Minis, Nanjing 210095, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Integrated Pest Management Ctr Hebei Prov, Inst Plant Protect, Key Lab IPM Crops No Region North China,Minist Ag, Baoding 071000, Peoples R China
关键词： Bacillus amyloliquefaciens; biofilm formation; drought tolerance; stomatal aperture; abscisic acid
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2019 年 20 卷 24 期
页码：
收录情况： SCI
摘要： Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus amyloliquefaciens 54, from 30 potential PGPR to induce drought tolerance in tomato plants was examined via greenhouse screening. The results indicated that B. amyloliquefaciens 54 significantly enhanced drought tolerance by increasing survival rate, relative water content and root vigor. Coordinated changes were also observed in cellular defense responses, including decreased concentration of malondialdehyde and elevated concentration of antioxidant enzyme activities. Moreover, expression levels of stress-responsive genes, such as lea, tdi65, and ltpg2, increased in B. amyloliquefaciens 54-treated plants. In addition, B. amyloliquefaciens 54 induced stomatal closure through an abscisic acid-regulated pathway. Furthermore, we constructed biofilm formation mutants and determined the role of biofilm formation in B. amyloliquefaciens 54-induced drought tolerance. The results showed that biofilm-forming ability was positively correlated with plant root colonization. Moreover, plants inoculated with hyper-robust biofilm (Delta abrB and Delta ywcC) mutants were better able to resist drought stress, while defective biofilm (Delta epsA-O and Delta tasA) mutants were more vulnerable to drought stress. Taken altogether, these results suggest that biofilm formation is crucial to B. amyloliquefaciens 54 root colonization and drought tolerance in tomato plants.
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