您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Functional characterization of one sex pheromone receptor (AlucOR4) in Apolygus lucorum (Meyer-Dur)

文献类型： 外文期刊

作者： An, Xingkui 1 ; Khashaveh, Adel 1 ; Liu, Danfeng 1 ; Xiao, Yong 1 ; Wang, Qi 1 ; Wang, Shanning 2 ; Geng, Ting 3 ; Gu, Sh 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

3.Chinese Acad Agr Sci, Langfang Sci Res Trial Stn, Langfang 065000, Peoples R China

关键词： Apolygus lucorum; Sex pheromone receptor; AlucOR4; Xenopus oocyte recording; RNAi; Electroantennogram (EAG)

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN： 0022-1910

年卷期： 2020 年 120 卷

页码：

收录情况： SCI

摘要： Traps baited with female-produced sex pheromones have been very effective in the monitoring and management of mirid bugs in numerous field trials. However, none of the target odorant receptors for sex pheromone components in Apolygus lucorum have been identified. Here, we identified one candidate sex pheromone receptor, AlucOR4, from A. lucorum. Quantitative real-time PCR (qPCR) analysis revealed that AlucOR4 was antennae-enriched and male-biased in adult A. lucorum. Xenopus oocyte expression system assays demonstrated that AIucOR4/AlucOrco was sensitive to two major sex pheromone constituents and exhibited high sensitivity to (E)2-hexenyl butyrate (E2HB) and lower sensitivity to hexyl butyrate (HB). The expression level of target mRNA was significantly reduced ( > 80%) in dsAlucOR4-injected bugs after five days. The electroantennogram (EAG) responses of male antennae to E2HB and HB were also reduced significantly (similar to 40%). Our findings suggest that AlucOR4 is essential to sex pheromone perception in A. lucorum.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台