文献类型： 外文期刊

作者： He, Yansen ¹ ; Xu, Min ² ; Li, Ruimei ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Inst Subtrop Agr, 1499 Jiulong Ave, Zhangzhou 363005, Fujian, Peoples R China

2.Hainan Univ, Inst Trop Agr & Forestry, Haikou, Hainan, Peoples R China

关键词： Narcissus flower colour; transcriptional level; carotenoids; flavonoids; chlorophylls; DEGs

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN： 1462-0316

年卷期：

页码：

收录情况： SCI

摘要： Chinese narcissus is a traditional flower with gorgeous corona and petal colour. The carotenoid biosynthesis pathway is primarily responsible for colour formation in narcissus flower. However, the role of carotenoid biosynthesis enzymes and intermediates in colour development, and the molecular basis of differences between orange-yellow corona and milky-white petals are unclear. Here, we performed RNA-Seq using Illumina HiSeq platform and de novo transcriptome assembly to predict the functions of the identified significantly differentially expressed genes (DEGs). We investigated genes expression in narcissus corona and petal tissues on the first and seventh day of flowering. 14 colour -related genes were further analysed by qRT-PCR. The significantly DEGs encoded various kinds of enzymes and transcription factors (TFs) associated with carotenoid and flavonoid biosynthesis pathways, respectively. These DEGs mainly included phytoene synthase (PSY) gene, lycopene epsilon cyclase (LCYE) gene, beta-carotene hydroxylase (BCH) gene, beta-carotene isomerase gene and AP2/ ERF, B3, MYB, NAC and WRKY TFs. Additionally, an in-depth analysis of chlorophyll biosynthesis and degradation pathways was performed. These results enhance our understanding of the mechanism of colour formation in narcissus flower and provide a basis for further investigation of the functions of proteins encoded by the annotated unigenes and cDNA sequences of narcissus.