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Identification and characterization of novel antioxidant peptides from crucian carp (Carassius auratus) cooking juice released in simulated gastrointestinal digestion by UPLC-MS/MS and in silico analysis

文献类型： 外文期刊

作者： Zhang, Jin 1 ; Du, Hongying 1 ; Zhang, Gaonan 1 ; Kong, Fanbin 4 ; Hu, Yang 1 ; Xiong, Shanbai 1 ; Zhao, Siming 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China

3.Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Hubei, Peoples R China

4.Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA

5.Ctr Convent Freshwater Fish Proc, Natl R&D Branch, Wuhan 430070, Hubei, Peoples R China

关键词： Antioxidant peptides; Crucian carp; Gastrointestinal digestion; In silico assessment; RP-HPLC; UPLC-MS/MS

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

ISSN： 1570-0232

年卷期： 2020 年 1136 卷

页码：

收录情况： SCI

摘要： The objective of this work was to separate, identify and assess antioxidant peptides from the simulated gastrointestinal (GI) digestion of crucian carp (Carassius auratus) cooking juice (CCCJ), which has been previously found with this activity. The CCCJ after simulated GI digestion treatment was separated gradually by ultrafiltration and RP-HPLC. Five novel antioxidant peptides with 10-13 amino acid residues were identified by UPLC-MS/MS. Their in silico assessments showed amphiphilic nature, good sensory quality and different target sites in the human body. Meanwhile, their three-dimensional structure predictions exhibited at least one beta-turn, beta-sheet and/or alpha-helix with partial hydrophobic and/or net-charged residues exposed to the external medium, which was good evidence for high antioxidant activity. Ultimately, four novel peptides with high antioxidant activity were found, among which IREADIDGDGQVN (1401 Da), PEILPDGDHD (1107 Da) and ASDEQDSVRL (1119 Da) exerted the highest DPPH radical scavenging activity with IC50 of 1.78, 1.18 and 1.45 mM, respectively, while APLEEPSSPH (1063 Da) showed the highest Fe2+ chelating ability with IC50 of 0.09 mM. This work could help understand the mechanism of CCCJ on human health promotion and improve the economic value of the crucian carp processing industry.

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