您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Polycalin is involved in the toxicity and resistance to Cry1Ac toxin in Helicoverpa armigera (Hubner)

文献类型： 外文期刊

作者： Wang, Bingjie 1 ; Wei, Jizhen 3 ; Wang, Yanan 2 ; Chen, Lin 2 ; Liang, Gemei 2 ;

作者机构： 1.Minist Agr & Rural Affairs, Environm & Plant Protect Inst, Chinese Acad Trop Agr Sci, Key Lab Integrated Pest Management Trop Crops, Haikou, Hainan, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Henan Agr Univ, Coll Plant Protect, State Key Lab Wheat & Maize Crop Sci, Zhengzhou, Peoples R China

关键词： functional receptor; Helicoverpa armigera; polycalin; resistance mechanisms

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2020 年 104 卷 1 期

页码：

收录情况： SCI

摘要： Polycalin has been confirmed as a binding protein of the Cry toxins in a few Lepidoptera insects, but its function in the action mechanism of Cry1Ac and whether it is involved in resistance evolution are still unclear. In this study, Ligand blot and enzyme-linked immunosorbent assays showed that Helicoverpa armigera polycalin could specifically interact with Cry1Ac with a high affinity (K-d = 118.80 nM). Importantly, antisera blocking polycalin in H. armigera larvae decreased the toxicity of Cry1Ac by 31.84%. Furthermore, the relative gene and protein expressions were lower in Cry1Ac-resistant strain (LF60) than that in Cry1Ac-susceptible strain (LF). These findings indicated that H. armigera polycalin was a possible receptor of Cry1Ac and may be contributed to the resistance to Cry1Ac.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台