文献类型： 外文期刊

作者： Fu, X. J. ¹ ; Pei, J. X. ¹ ; Zheng, Yt ¹ ; Guo, D. D. ¹ ; Yang, Q. H. ¹ ; Jin, H. X. ¹ ; Zhu, D. H. ¹ ; Dong, O. K. ¹ ; Xu, ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Hangzhou Natl Sub Ctr Soybean Improvement, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

关键词： HRM; SNP fingerprint; Soybean; Zhexian No.9

期刊名称：LEGUME RESEARCH （ 影响因子：0.589； 五年影响因子：0.648 ）

ISSN： 0250-5371

年卷期： 2020 年 43 卷 1 期

页码：

收录情况： SCI

摘要： Single nucleotide polymorphisms (SNPs) have been proved to be powerful markers in genetic analysis due to their high abundance and polymorphism in plant genomes. The recently developed high-resolution melting (HRM) analysis method provides a novel, quick, and close-tube PCR approach to analyze SNP variations. In present study, 101 HRM-based SNP markers from 20 soybean chromosomes were developed for genotyping vegetable soybean cultivar 'Zhexian No.9' with 'Williams 82' as reference. 33.7% of these markers were polymorphic between 'Zhexian No.9' and 'Williams 82'. Polymorphic markers were found on 85% (17 of 20) of the soybean chromosomes when comparing 'Zhexian No. 9' and 'Williams 82'. Finally, an array of 101 in-sequence nucleotide letters was generated as the first precise SNP fingerprint of 'Zhexian No.9'. The described marker-developing methodology could be used in other crops with known genomic information.