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Regulation of 14-3-3 beta/alpha gene expression in response to salinity, thermal, and bacterial stresses in Siberian sturgeon (Acipenser baeri)

文献类型： 外文期刊

作者： Wang, Xiaowen 1 ; Ma, Guoqing 1 ; Zhu, Hua 1 ;

作者机构： 1.Beijing Fisheries Res Inst & Beijing Key Lab Fish, Beijing, Peoples R China

2.Natl Freshwater Fisheries Engn Technology Res Ctr, Beijing, Peoples R China

关键词： Siberian sturgeon; 14-3-3 beta; alpha; Salinity stress; Thermal stress; Bacterial challenge

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2020 年 46 卷 2 期

页码：

收录情况： SCI

摘要： The 14-3-3 proteins are a family of widely expressed acidic proteins, which are involved in the regulation of many biological processes of animals. However, no research regarding 14-3-3 has been described in sturgeon to date, one of the most primitive Actinopterygii species. Here, we identified the first 14-3-3 gene from Siberian sturgeon (Acipenser baeri), named Ab14-3-3 beta/alpha (GenBank Accession No. KY094076.1). The cDNA of Ab14-3-3 beta/alpha is 1212 bp in length, containing a 5 '-untranslated region (UTR) of 82 bp, a 3 ' UTR of 392 bp, and an open reading frame (ORF) of 738 bp, encoding a polypeptide of 245 amino acids which contains a 14-3-3 homologs domain (PF00244). Phylogenetic analysis showed that the 14-3-3 gene product from Acipenser baeri is a counterpart of vertebrate 14-3-3 beta/alpha. The deduced Ab14-3-3 beta/alpha protein shares high identities of 46.5-95.5% with the homologs of other species. Ab14-3-3 beta/alpha mRNA was constitutively expressed in all examined tissues, with high expression levels in the blood and gill. Furthermore, the expression level of Ab14-3-3 beta/alpha mRNA increased significantly in the gill at 1 h under acute salinity shock by transfer of Siberian sturgeons from fresh water (FW) to 15 ppt. In fish subjected to a high temperature (31 degrees C), Ab14-3-3 beta/alpha showed a significant upregulation in the liver at 3 h compared with the control group (24 degrees C). A 4.85-fold increase of Ab14-3-3 beta/alpha expression in the spleen of Siberian sturgeon was observed at 24 h following Aeromonas hydrophila challenge. Collectively, these results indicated that Ab14-3-3 beta/alpha might play a certain role in sturgeon in response to some environmental stresses and bacterial challenge.

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