Development of chromatographic fingerprint for quality analysis of diploid and tetraploid Lonicera japonica
文献类型： 外文期刊
作者： Hu Xuan 1 ; Wang Dan 1 ; Pang Yuxin 1 ; Wu Zhisheng 2 ; Huan Hengfu 1 ; Chen Zhijian 1 ; Li Weidong 2 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Crop Genet Resources, Danzhou 571737, Peoples R China
2.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China
关键词： Lonicera; Polyploidy; Chromatography, high pressure liquid; Dermatoglyphics; Phytochemicals; Qualitative research
期刊名称：JOURNAL OF TRADITIONAL CHINESE MEDICINE （ 影响因子：0.848； 五年影响因子：1.32 ）
ISSN： 0255-2922
年卷期： 2020 年 40 卷 1 期
页码：
收录情况： SCI
摘要： OBJECTIVE: To develop the chromatographic fingerprint of Lonicera japonica (L. japonica) and evaluate the effects of polyploidy on the quality of L. japonica. METHODS: High-performance liquid chromatography (HPLC) methods used to establish the chromatographic fingerprint were developed. The quality of 11 batches of diploid L. japonica and 13 batches of tetraploid L. japonica collected from different regions across China were analyzed. The contents of five active compounds, consisting of chlorogenic acid, rutin, galuteolin, isochlorogenic acid A and quercetin, were further detected in L. japonica. RESULTS: The chromatographic fingerprint established by the optimized HPLC method was verified for qualitative analysis of L. japonica. Quantitative analysis showed that the contents of chlorogenic acid, isochlorogenic acid A, and quercetin in tetraploid plants were higher than those in diploid plants, whereas, rutin and galuteolin contents in tetraploid plants were lower than those in diploid plants. CONCLUSION: The developed HPLC method is suitable for qualitative analysis of L. japonica. Polyploidy was indicated to influence the chemical properties of L. japonica. Tetraploid L. japonica shows potential for utilization as a medicinal plant with different active components. (C) 2020 JTCM. All rights reserved.
- 相关文献
