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SRNAome and transcriptome analysis provide insight into strawberry fruit ripening

文献类型: 外文期刊

作者: Wang, Yunxiang 1 ; Li, Wensheng 1 ; Chang, Hong 1 ; Zhou, Jiahua 1 ; Luo, Yunbo 5 ; Zhang, Kaichun 1 ; Zuo, Jinhua 6 ; Wa 1 ;

作者机构: 1.Beijing Acad Agr & Forestry Sci, Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China

2.Natl R&D Ctr Fruit Proc, Beijing 100093, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100093, Peoples R China

4.Beijing Engn Res Ctr Deciduous Fruit Trees, Beijing 100093, Peoples R China

5.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

6.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Vegetable Postharvest Proc, Minist Agr, Beijing 100097, Peoples R China

关键词: Strawberry fruit ripening; miRNAs; Transcriptome; Degradome analysis

期刊名称:GENOMICS ( 影响因子:5.736; 五年影响因子:4.939 )

ISSN: 0888-7543

年卷期: 2020 年 112 卷 3 期

页码:

收录情况: SCI

摘要: Strawberry fruit ripening is a complex process affected by multiple factors at different regulation levels. To elucidate the regulation mechanisms, the combined analysis of sRNAome and transcriptome were used. A total of 124 known and 190 novel miRNAs were found, 62 of them were significantly differentially expressed (DE). The targets of the DE miRNAs were parsed and several TFs, such as SPL, ARF, WRKY, and TCP, were found to be involved in ripening. Elevated CO 2 can significantly postpone ripening and miR156, miR166f, miR171a, and miR171d were the DE miRNAs. Transcriptome analysis found 313 DE genes related to fruit ripening, including cell wall metabolism-related genes, color-related genes, ethylene-related genes, and genes encoding TFs such as MYB, SPL, NAC, TCP, and ARF. Based on above, a combined regulatory model involved in fruit ripening was created. These results provide valuable information for understanding the complicated coordinated regulatory network of strawberry fruit ripening.

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