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Molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-I in duck (Anas platyrhynchos)

文献类型： 外文期刊

作者： Gu, Tiantian 1 ; Li, Guoqin 2 ; Tian, Yong 2 ; Chen, Li 2 ; Wu, Xinsheng 1 ; Zeng, Tao 2 ; Xu, Qi 1 ; Vladyslav, Spyrydon 1 ;

作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Anim Genet Breeding & Mol Design, Yangzhou 225009, Jiangsu, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310021, Zhejiang, Peoples R China

3.Minist Agr China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

4.Natl Univ Life & Environm Sci Ukraine, UA-03041 Kiev, Ukraine

关键词： duck; retinoic acid-inducible gene I; gene expression; antivirus; poly(I; C); Anas platyrhynchos

期刊名称：JOURNAL OF GENETICS （ 影响因子：1.166； 五年影响因子：1.212 ）

ISSN： 0022-1333

年卷期： 2020 年 99 卷 1 期

页码：

收录情况： SCI

摘要： Intracellular double-stranded RNA (dsRNA) is a chief sign of replication for many viruses. Pattern recognition receptors (PRRs) of the innate immune system detected the dsRNA and initiate the antiviral responses. Retinoic acid-inducible gene I (RIG-I), a member of PRRs, plays an essential regulatory role in dsRNA-induced signalling. In this study, the full-length complementary DNA (cDNA) of duck RIG-I (duRIG-I) was cloned using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA of duRIG-I contained 97-bp 5 ' UTR, 141-bp 3 '-UTR and 2802 bp complete open-reading frame (ORF) encoding 933 amino acids. Multiple sequence alignments showed that duRIG-I shared high similarity with RIG-I from other vertebrates. Quantitative real-time PCR (qRT-PCR) analysis revealed that duRIG-I mRNA was expressed in all tested tissues, with high levels in the liver, heart, spleen, kidney and thymus, while lower in the duodenum. duRIG-I could be induced by treatment with poly(I:C). Further, overexpression of duRIG-I significantly activated the transcription of poly(I:C)-induced IFN-beta, IRF7, TRIF, Mx, STAT1 and STAT2 mRNA, and duRIG-I knockdown showed the opposite results. Overall, our results suggested that duRIG-I could be an important receptor for mimicking antiviral state in duck, which warrant further studies to show the possible mechanism.

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