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Heteroexpression of Osa-miR319b improved switchgrass biomass yield and feedstock quality by repression of PvPCF5

文献类型： 外文期刊

作者： Liu, Yanrong 1 ; Yan, Jianping 2 ; Wang, Kexin 2 ; Li, Dayong 4 ; Han, Yejun 5 ; Zhang, Wanjun 2 ;

作者机构： 1.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Grassland Sci & Technol, Beijing 100193, Peoples R China

3.China Agr Univ, Natl Energy R&D Ctr Biomass NECB, Beijing 100193, Peoples R China

4.Beijing Acad Agr & Forestry Sci, BVRC, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China

5.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China

关键词： Switchgrass; MiR319; PvPCF5; Lignin; Biomass yield

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN：

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Background Switchgrass (Panicum virgatum L.), a C-4 perennial grass, has been recognized as one of the most potentially important lignocellulose biofuel crops. MicroRNA319 (miR319) plays a key role in plant development, abiotic resistance, and cell wall biosynthesis by repressing expression of its target TCP genes. We hypothesized miR319-TCP pathway could play important roles in switchgrass feedstock characteristics for biofuel production, and produced switchgrass transgenic plants overexpressing miR319 (by ectopic expressing Osa-MIR319b gene), blocking miR319 (by overexpressing a target mimicry of miR319/MIM319) and repression of miR319 target gene PvPCF5. Plant phenotype, biomass yield, and feedstock quality of transgenic plants were analyzed. Results Overexpression of miR319 in switchgrass promoted leaf elongation and expansion of transgenic plants, increased plant height, stem diameter, and resulted in a significant increase in plant biomass yield. Transgenic plants overexpressing of miR319 reduced lignin content, showed significantly higher enzymatic hydrolysis efficiency compared to the wild type plant. However, opposite results were observed in the MIM319 plants. Furthermore, suppression of miR319 target gene PvPCF5 activity also reduced lignin content, increased lignin monomer S/G ratio and the proportion of beta-O-4 linkages, while significantly improving the sugar production per plant. Quantitative real-time (qRT-PCR) analysis indicated that expression of PvMYB58/63B and PvHCT with predicted TCP binding sites in their promoter regions was negatively regulated by miR319-PvPCF5 module. Conclusions MiR319-PvPCF5 module plays positive roles in regulating biomass yield and quality of switchgrass. It can be utilized as a candidate molecular tool in regulating biomass yield and feedstock quality. The finding could also be transferred to other grasses for forage quality improvement through genetic manipulation.

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