Whole-Genome Analysis of Two Copies of bla(NDM-1) Gene Carrying Acinetobacter johnsonii Strain Acsw19 Isolated from Sichuan, China
文献类型： 外文期刊
作者： Tang, Lingtong 1 ; Shen, Wei 2 ; Zhang, Zhikun 2 ; Zhang, Jingping 2 ; Wang, Guangxi 2 ; Xiang, Li 2 ; She, Junping 2 ; H 1 ;
作者机构： 1.Peoples Hosp Gao Cty, Dept Clin Lab, Yibin 644000, Sichuan, Peoples R China
2.Southwest Med Univ, Sch Basic Med, Dept Pathogen Biol, 319,Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China
3.First Peoples Hosp Yibin, Dept Clin Lab, Yibin 644000, Sichuan, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Inst Plant Nutr & Resources, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China
关键词： Acinetobacter johnsonii; bla(NDM-1); bla(OXA); genomic island
期刊名称：INFECTION AND DRUG RESISTANCE （ 影响因子：4.003； 五年影响因子：4.323 ）
ISSN： 1178-6973
年卷期： 2020 年 13 卷
页码：
收录情况： SCI
摘要： Purpose: To characterize the genetic feature of the carbapenems resistant Acinetobacter johnsonii strain Acsw19 isolated from municipal sludge. This strain was found to carry two copies of bla(NDm-1), cmlB1-like gene, and bla(OXA-211)-like gene along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages. Methods: A carbapenem-resistant Acinetobacter johnsonii strain Acsw19 isolated from municipal sludge was subjected to whole-genome sequencing (WGS) via the PacBio and Illumina MiSeq platforms. Thereafter, the characteristic was analyzed by a series of bioinformatics software. Results: The results showed that the genome of Acsw19 was consisted of a 3,433,749 bp circular chromosome and 3 circular plasmids, pAcsw19-1 (11,161 bp), pAcsw19-2 (351,885 bp) and pAcsw19-3 (38,391bp), respectively. Resistome analysis showed that Acsw19 carried 12 antimicrobial resistance genes, including 6 [cmlB1-like, bla(NDM-1), bla(OXA-58), aph (3')-VIa, msr(E) and mph(E)] in the plasmid pAcsw19-2 and 6 (bla(OXA-211)-like, bla(NDM-1), aph(3 '')-Ib, aph(6)-Id, su12, and floR) in the chromosome genome. Specifically, the cmlB1-like gene shared 86.33%, 71.7% and 71.9% similarities with the cmlB1, cmlA4 and endA8 gene, and the bla(OXA-211)-like gene shared 94.4%, 95.39% and 96.36% similarities with bla(OXA-211), bla(OXA-643) and bla(OXA-652), at the nucleotide level, respectively. Phylogenetic analysis showed that the bla(OXA-211)-like gene and endBl-like gene had the closest evolutionary relationship with bla(OXA-)(643) and cmlB1, respectively. These results indicated that the bla(OXA-211)-like and cmlB1-like genes identified in the current study should be the novel variant resistance genes. Conclusion: Carrying of two copies of bla(NDM-1), cmlB1-like, bla(OXA-211)-like and along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages Acinetobacter johnsonii strain might increase the possibility of spreading of resistance genes.
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