Normal expression of insect-resistant transgene in progeny of common wild rice crossed with genetically modified rice: its implication in ecological biosafety assessment
文献类型： 外文期刊
作者： Xia, Hui 1 ; Lu, Bao-Rong 1 ; Su, Jun 2 ; Chen, Rui 2 ; Rong, Jun 1 ; Song, Zhiping 1 ; Wang, Feng 2 ;
作者机构： 1.Fudan Univ, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Inst Biodivers Sci, Shanghai 200433, Peoples R China
2.Fujian Acad Agr Sci, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China
关键词： progeny;transgene outflow
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
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收录情况： SCI
摘要： Transgene outflow from genetically modified (GM) rice to its wild relatives may cause undesirable ecological consequences. Understanding the level of transgene expression in wild rice following gene flow is important for assessing such consequences, providing that transgene escape from GM rice cannot be prevented. To determine the expression of a transgene in common wild rice (Oryza rufipogon), we analyzed the content of Cry1Ac protein in three GM rice lines containing a Bt transgene, their F-1 hybrids with common wild rice and F-2 progeny at different growth stages, using the sandwich enzyme-linked immunosorbent assay. The average content of Cry1Ac protein in leaf samples of the wild rice lines ranged between 0.016 and 0.069% during the entire growth period, whereas that in stems varied between 0.12 and 0.39%. A great variation in Cry1Ac protein content was detected among individuals of F-1 hybrids and F-2 progeny, with some wild individuals showing higher level of Bt toxin than the cultivated GM rice. The results suggest that the Bt transgene can express normally in the interspecific hybrids between insect-resistant GM rice and common wild rice, and may have similar effects on the target insects as in GM rice.
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