文献类型： 外文期刊

作者： Zhang, Ai-Lian ¹ ; Luo, Jin-Xian ² ; Zhang, Tian-Yuan ² ; Pan, Ying-Wen ¹ ; Tan, Yan-Hua ¹ ; Fu, Ce-Yi ⁴ ; Tu, Fa-zhi ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Sun Yat Sen Univ, Key Lab Gene Engn, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

3.Sun Yat Sen Univ, Dept Biochem, Guangzhou 510275, Guangdong, Peoples R China

4.Hainan Prov Inst Drug Control, Haikou 570216, Hainan, Peoples R China

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins and the most important feature of P. pastoris is the existence of a strong and tightly regulated promoter from the alcohol oxidase I (AOX1) gene. The AOX1 promoter (pAOX1) has been used to express foreign genes and to produce a variety of recombinant proteins in P. pastoris. However, some efforts have been made to develop new alternative promoters to pAOX1 to avoid the use of methanol. The glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP) has been used for constitutive expression of many heterologous proteins. The pGAP-based expression system is more suitable for large-scale production because the hazard and cost associated with the storage and delivery of large volume of methanol are eliminated. Some important developments and features of this expression system will be summarized in this review.