Micropropagation of self-rooting juvenile clones by secondary somatic embryogenesis in Hevea brasiliensis
文献类型: 外文期刊
作者: Hua, Y. W. 1 ; Huang, T. D. 1 ; Huang, H. S. 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Minist Agr, Rubber Res Inst, Key Lab Rubber Biol, Danzhou 571737, Hainan, Peoples R China
关键词: Hevea brasiliensis;secondary somatic embryogenesis;self-rooting juvenile clone;embryo multiplication With 2 figures and 5 tables
期刊名称:PLANT BREEDING ( 影响因子:1.832; 五年影响因子:1.956 )
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收录情况: SCI
摘要: With 2 figures and 5 tables Micropropagation of self-rooting juvenile clones in Hevea brasiliensis was established for two clones CATAS 7-33-97 and CATAS 88-13 through the following three steps: induction of primary embryos, embryo multiplication by secondary somatic embryogenesis in three successive cycles from a single culture of primary embryo and plant regeneration. The embryo multiplication coefficients of the two clones increased in the first cycle and reached the maximum in the second and the third cycle at the same rate. Significant effects of origins of embryo fragments and calcium on secondary embryogenesis were detected, the highest ratios of the regenerated embryos to primary embryos appeared, when embryo fragments close to the base of embryos were used and incubated in Murashige and Skoog (MS)-based callogenesis medium with 6.0 m m CaCl for CATAS 88-13 and 9.0 m m CaCl for CATAS 7-33-97. The highest rates of plant conversion were produced on MS-based plant regeneration medium with 4.5 and 9.0 o m 2,4-D for CATAS 7-33-97 (85.0%) and 13.5 o m for CATAS 88-13 (75.0%), being higher than other reports (60%). Finally, the application of this system was discussed.
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