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Noninvasive Expressions of ipt in Whole Plants or Roots through pOp/LhG4 Indicate a Role of Plant Aerial Parts and Light in Cytokinin Synthesis and Root Inhibition

文献类型： 外文期刊

作者： Guo, Jianchun 1 ; Hu, Xinwen 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, State Key Lab Trop Crops Biotechnol, Haikou 571101, Peoples R China

2.Hainan Univ, Haikou 571104, Peoples R China

关键词： Noninvasive expression;Cytokinin synthesis;Constitutive expression;Root-specific expression;ipt Gene;pOp/LhG4

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

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收录情况： SCI

摘要： To study plant roots in response to ipt gene activation, the transcriptional fusions of ipt-GUS and GUS-ipt were expressed in roots or in whole plants of Arabidopsis under the control of a root-specific promoter TobRT7 or a CaMV35S promoter through the pOp/LhG4 system in noninvasive conditions. The transgenic plants with constitutive expression of ipt-GUS or GUS-ipt showed 15-25-fold or 1-2-fold increased cytokinin levels, respectively. ipt-GUS-expressing Arabidopsis had severe root inhibition, enlarged shoot apical parts, serrated leaves, and no or few sterile flowers, whereas GUS-ipt-expressing Arabidopsis grew faster, flowered early, and had more lateral shoots. However, when ipt-GUS and GUS-ipt were specially expressed in roots under the control of TobRT7, neither cytokinin content in roots or shoots nor phenotypes were altered. In cytokinin-overproducing, ipt-GUS-expressing Arabidopsis, the light and aerial parts of plants played an important role for cytokinin synthesis and root inhibition, and the ipt gene was vigorously expressed at the shoot apical parts. Meanwhile, calli were induced at the shoot apical parts of some cytokinin-overproducing, ipt-GUS-expressing Arabidopsis.

  • 相关文献

[1]Non-invasive expressions of ipt in whole plants or roots indicate cytokinins are synthesized in plant aerial parts, and coordinate with light affect on the phenotypes and the formation of anthocyanins, lignins. Guo, Jian-Chun,Hu, Xin-Wen. 2010

[2]Constitutive expression of barley alpha-amylase in Pichia pastoris by high-density cell culture. Liu, Z. W.,Yin, H. X.,Yi, X. P.,Zhang, A. L.,Zhang, Z. H.,Shen, J. C.,Chen, L. P.,Luo, J. X.,Zhang, T. Y.,Luo, J. X.,Zhang, T. Y.,Fu, C. Y..

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