文献类型： 外文期刊

作者： Wang, YH ¹ ; Wu, ZY ² ; Zhang, XH ² ; Chen, GQ ² ; Wu, Q ³ ; Huang, CL; Yang, Q;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China; Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China; Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China; Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China; Tsing Hua Univ, Dept Biol Sci & Biotech

关键词： medium-chain-length-polyhydroxyalkanoates;chloroplast genetic engineering;transgenic tobacco;plant bioreactor

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Medium-chain-length-polyhydroxyalkanoates (mcl-PHAs) belong to the group of microbial polyesters containing monomers ranging from 6 to 14 carbons in length. The key enzymes of their biosynthesis are PHA-polymerase (product of phaC gene) and 3-hydroxyacyl-acyl carrier pro-tein-CoA transferase (product of phaG gene). With aadA (aminoglycoside 3′-adenylyltransferase) gene as screening marker, two chloroplast transformation vectors of pTC2 harboring phaC2 gene only and pTGC harboring both phaC and phaG genes were constructed and introduced into tobacco chloroplast genome through particle bombardment. PCR and Southern blot analysis confirmed the insertion of the introduced genes into chloroplast genome. The content of mcl-PHAs accumulated in transgenic plants was analyzed by gas chromatography, mcl-PHAs accumulated up to 4.8 mg/g dry weight (dw) in transgenic line S4-3; their monomers were 3-hydroxyoctanoate and 3-hydroxydecanoate. Accumulation of mcl-PHAs polymers in the tobacco chloroplast was also observed by transmission electron microscopy. To our knowledge, this is the first report on the synthesis of mcl-PHAs in tobacco via chloroplast genetic engineering.