文献类型： 外文期刊
作者： Yan, Fei 1 ; Peng, Jiejun 1 ; Lu, Yuwen 1 ; Lin, Lin 1 ; Zheng, Hongying 1 ; Chen, Hairu 2 ; Chen, Jianping 1 ; Adams, Mi 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
2.Yunnan Agr Univ, Plant Protect Coll, Kunming 650205, Peoples R China
3.Rothamsted Res, Dept Plant Pathol & Microbiol, Harpenden AL5 2JQ, Herts, England
期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： In Arabidopsis thaliana, Dicer-like protein 2 (DCL2) cleaves double-stranded virus RNA, playing an essential role in the RNA interference pathway. Here, we describe three alternative splicing (AS) forms of AtDCL2: in one, both intron 8 and intron 10 are retained in the mRNA, in second only intron 8 is retained and in the third no intron is retained, but there is a deletion of 56 nucleotides at the end of exon 10. These splicing forms are present in stems and leaves at different development stages. AS was also detected in DCL2 of Brassica rapa, where intron 9, but not intron 8 or intron 10, was retained suggesting that AS may be a common phenomenon in cruciferous plant DCL2s. The retained introns and sequence deletions detected in AtDCL2 changed the reading frame and produced premature terminal codons. The AS forms appeared to be substrates of nonsense-mediated decay of mRNA.
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