您好，欢迎访问福建省农业科学院 机构知识库！
福建省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Rapid Integrated Detection Platform for Genes Related to Duck Muscle Tissue Development

文献类型： 外文期刊

作者： Zhu, Zhiming 1 ; Li, Li 1 ; Miao, Zhongwei 1 ; Xin, Qingwu 1 ; Zhang, Linli 1 ; Huang, Qinlou 1 ; Zheng, Nengzhu 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Prov Key Lab Livestock & Poultry Genet Bree, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Anim Sci, Coll Bee Sci, Fuzhou 350002, Fujian, Peoples R China

关键词： POCT; RT-qPCR; Duck; Muscle Development; Rapid Integrated Detection

期刊名称：JOURNAL OF BIOMEDICAL NANOTECHNOLOGY （ 影响因子：2.9； 五年影响因子：3.2 ）

ISSN： 1550-7033

年卷期： 2023 年 19 卷 12 期

页码：

收录情况： SCI

摘要： In duck breeding, the growth and development of skeletal muscle is an important factor influencing the meat production performance of ducks. Therefore, the determination of Myod and Myf5 gene expression in poultry skeletal muscle tissues can help to understand the muscle development of poultry, improve the production performance and feed conversion rate of animal organism, enhance the rapid protein deposition in animal organism, and obtain high quality and quantity of animal livestock products. In this study, a fluorescent PCR assay system for Myod and Myf5 genes was developed, and a dual integrated rapid detection platform suitable for detecting Myod and Myf5 genes in poultry muscle tissue with a sen-sitivity of 10 copies/mu L was constructed using a set of commercial, fully automated nucleic acid analyzer with integrated detection based on magnetic bead method for nucleic acid extraction and PCR fluorescence detection. For 20 simulated samples, the integrated detection system was consistent with the results of qPCR experiments after conventional labora-tory extraction, while the closed cassette-based detection reduced the chance of contamination occurrence, making the IP: 2038 10920 O Tue, 05 D 2023 104522 results more reliable and accurate, which is ideal for immediate on-site rapid detection. Copyright: American Scientific Publishers

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：福建省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台