Isolation and identification of a subtype C avian metapneumovirus in chickens in Jiangsu, China
文献类型： 外文期刊
作者： Yao, Zhiyan 1 ; Li, Jingyi 1 ; Shi, Yongyan 1 ; Sun, Tong 1 ; Yang, Xiaoyu 1 ; Mao, Jingyu 1 ; Quan, Rong 3 ; Wang, Dan 3 ; Wang, Dedong 1 ; Zhou, Jianwei 1 ; Liu, Jue 1 ; Chu, Jun 4 ; Hou, Lei 1 ;
作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou, Peoples R China
2.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing, Peoples R China
4.Beijing Univ Agr, Coll Anim Sci & Technol, 7 Beinong Rd, Beijing 102206, Peoples R China
关键词： Avian metapneumovirus subgroup C (aMPV/C); Isolation and identification; Nucleoprotein (N) gene; Phylogenetic analysis; Pathogenicity
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）
ISSN： 0882-4010
年卷期： 2025 年 202 卷
页码：
收录情况： SCI
摘要： Avian metapneumovirus (aMPV) is a viral pathogen that mainly causes respiratory signs and drops in egg production in turkeys, chickens, and ducks. Here, an aMPV subgroup C (aMPV/C) strain, designated GX22-01, was isolated and identified from severe respiratory disease in broiler breeder chickens in 2022 in Jiangsu, China, as evidenced by indirect immunofluorescence and western blotting using specific anti-viral protein antibodies and by sequence analysis of viral nucleoprotein (N) gene. N gene sequencing indicated that the GX22-01 strain shares a high identity (94.3%-99.8 %) with aMPV/C isolates, especially with Chinese aMPV/C isolates from ducks and chickens, which are divided into aMPV/C cluster through N gene-based phylogenetic analysis. The aMPV/C GX22-01 strain was continuously passaged in Vero cells and the viral titers approximately reached 106.0 TCID50/0.1 mL. Pathogenic analysis showed that aMPV/C GX22-01 strain inoculation caused respiratory signs in 2-week-old specific-pathogen-free (SPF) chickens and resulted in pathogenic damage in tracheae and lung tissues, accompanied by positive viral signals using indirect immunohistochemistry. These results provide epidemiological and pathogenic data for developing effective measures against aMPV/C infection in China.
- 相关文献
作者其他论文 更多>>
-
Ozone treatment increase the whiteness of soy protein isolate through the degradation of isoflavone
作者：Li, Junyou;Chen, Yunqi;Yin, Lijun;Lv, Chenyan;Zang, Jiachen;Zhao, Guanghua;Zhang, Tuo;Wang, Dan;Yin, Lijun;Zhao, Guanghua;Zhang, Tuo
关键词：Soy protein isolate; Ozone; Soy isoflavones; Color mechanism; Structure and functional properties
-
Seneca Valley virus 3C protease cleaves HDAC4 to antagonize type I interferon signaling
作者：Li, Zijian;Yang, Jingjing;Ma, Ruiyi;Xie, Shijie;Wang, Dan;Quan, Rong;Song, Jiangwei;Li, Zijian;Yang, Jingjing;Wen, Xuexia;Liu, Jue
关键词：Seneca Valley virus (SVV); HDAC4; 3C protease; cleavage; type I interferon (IFN-I)
-
The Seneca Valley virus 3C protease cleaves DCP1A to attenuate its antiviral effects
作者：Yang, Jingjing;Li, Zijian;Wen, Xuexia;Yang, Jingjing;Li, Zijian;Wen, Xuexia;Ma, Ruiyi;Xie, Shijie;Wang, Dan;Quan, Rong;Song, Jiangwei
关键词：Seneca Valley virus (SVV); DCP1A; 3C protease; 3D; cleavage
-
A deep learning-based micro-CT image analysis pipeline for nondestructive quantification of the maize kernel internal structure
作者：Wang, Juan;Liu, Gui;Zhao, Chunjiang;Wang, Juan;Yang, Si;Wang, Chuanyu;Wen, Weiliang;Zhang, Ying;Guo, Xinyu;Zhao, Chunjiang;Wang, Juan;Yang, Si;Wang, Chuanyu;Wen, Weiliang;Zhang, Ying;Guo, Xinyu;Li, Jingyi
关键词：Maize kernel; Vitreous endosperm; Starchy endosperm; Semantic segmentation; Mirco-CT
-
Comparative efficacy and safety of therapeutic strategies for EGFRvIII positive recurrent glioblastoma
作者：Qiu, Jinming;Zhu, Fangge;Ye, Haoyuan;Su, Wenting;Wang, Renxi;Fu, Yi;Quan, Rong
关键词：
-
Evaluation of the nutritional, in vitro protein digestive and bioactive characteristics of a quinoa-based protein beverage
作者：Li, Mengzhuo;Li, Mengzhuo;Wang, Junjuan;Zhao, Xiaoyan;Wang, Dan;Zhao, Yuanyan;Ge, Zhiwen;Qin, Peiyou;Zhu, Manli;Zhang, Lizhen;Zou, Liang;Qin, Peiyou
关键词：Quinoa; Plant-based protein beverage; Amino acid score; In vitro protein digestibility; Antioxidant activity; ACE inhibitory activity
-
Remotely estimate the cropland fractional vegetation cover using linear spectral mixture analysis and improved band operations
作者：Yao, Yihan;Shen, Jianing;Yue, Jibo;Shu, Meiyan;Fu, Yuanyuan;Qiao, Hongbo;Sun, Tong;Zheng, Guang;Liu, Yang;Feng, Haikuan;Liu, Yang
关键词：Fractional vegetation cover; remote sensing; linear spectral mixture analysis
