文献类型： 外文期刊

作者： Teng, Yantong ¹ ; Cai, Maohong ¹ ; Xie, Qinyu ¹ ; Liu, Qinglong ³ ; Zhang, Haiwen ⁴ ; Chen, Tao ¹ ;

作者机构： 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Zhejiang Prov Key Lab Genet Improvement & Qual Con, Hangzhou 311121, Zhejiang, Peoples R China

2.Inner Mongolia Univ, Coll Life Sci, Hohhot 010021, Inner Mongolia, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

4.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： Rice; BEAR1; Seedling growth; Gibberellins; OsKS4

期刊名称：CROP JOURNAL （ 2022影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 3 期

页码：

收录情况： SCI

摘要： The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development. In this study, we identified a new bHLH transcription factor, BEAR1, from rice RNAi mutant library. Both the BEAR1-RNAi and bear1 CRISPR mutants had accelerated seedling growth. Histological section of leaves showed accelerated development of lacuna and vascular bundles in bear1 mutant. GUS staining revealed that BEAR1 was highly expressed in coleoptiles and leaves at seedling stage. Expression analysis of gibberellin (GA) biosynthesis and metabolic genes and content determination of active GAs indicated that the expression of GA biosynthesis genes, especially OsKS4 and OsCPS2, were upregulated and the GAs content were significantly increased in bear1, which correlated with the seedling phenotype of bear1 mutant. Molecular and biochemical assays revealed that BEAR1 directly binds to the promoter of OsKS4, thereby repressing its expression. Haplotypes analysis showed clear differentiation in indica and japonica rice varieties, and a strong correlation with seedling height. These findings provide novel insights into the regulation of seedling growth in rice. & COPY; 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).