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Development of species-specific PCR detection for three Mycogone species causing wet bubble disease in white button mushroom

文献类型： 外文期刊

作者： Shi, Niu-Niu 1 ; Ruan, Hong -Chun 1 ; Chen, Wen -Le 3 ; Chen, Qiao-Hong 4 ; Chen, Fu-Ru 1 ; Du, Yi-Xin 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Key Lab Monitoring & Integrated Management, Fuzhou 350013, Fujian, Peoples R China

3.Sanming Plant Protect & Inspect Stn, Sanming 365001, Fujian, Peoples R China

4.Sanming Agr Reclamat Stn, Sanming 365001, Fujian, Peoples R China

关键词： Agaricus bisporus; Mycogone spp; Translation elongation factor 1?; Species -specific detection; Wet bubble disease

期刊名称：CROP PROTECTION （ 影响因子：2.8； 五年影响因子：3.0 ）

ISSN： 0261-2194

年卷期： 2023 年 164 卷

页码：

收录情况： SCI

摘要： Agaricus bisporus, commonly known as white button mushroom, is a major agricultural crop worldwide. How-ever, wet bubble disease in Agaricus bisporus, caused by three Mycogone species, namely Mycogone perniciosa, M. rosea and M. xinjiangensis, leads to tremendous economic losses in China. The three species are difficult to distinguish based on morphology and pathogenicity. An early, fast and accurate method to detect and differ-entiate among these species is essential for controlling the disease. Based on differences in Tef-1 alpha gene sequences, therefore, three sets of species-specific primers, T-6-3/AT-4, T-2/AT-2 and T-7-1/AT-3, were successfully designed for the detection and identification of M. perniciosa, M. rosea and M. xinjiangensis, respectively, using an endpoint PCR assay. Subsequently, the specificities of the three primers were demonstrated using 43 isolates, including Mycogone spp., A. bisporus, Trichoderma harzianum, Trichothecium roseum, Cladobotryum dendroides, Lecanicillium fungicola, Fusarium solani and other soil-borne pathogens. The detection limits for M. perniciosa, M. rosea and M. xinjiangensis were 0.5 pg/mu L, 0.2 ng/mu L and 20 pg/mu L of DNA template, respectively, suggesting strong amplification with the three primer pairs. Moreover, the species-specific primers efficiently detecteerd the target species in artificially inoculated mushrooms and soils. This is the first report to identify and distinguish each of the three Mycogone species. Besides, this PCR-based molecular method could provide a sensitive, quick and highly specific tool to discriminate among the three Mycogone species. Collectively, the assay developed in this study could have great application value for early detection of Mycogone species in soil and effective control of wet bubble disease.

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